In the enumeration of bacteria in a culture by a plate count, all of the following apply when determining the numbers except: Group of answer choices All bacteria in the culture are counted Statistically only plates with between 30-300 colonies should be counted It is assumed that each colony arose from division of one or a few cells All of the answer choices are correct The medium must contain nutrients used by the bacteria
Question
In the enumeration of bacteria in a culture by a plate count, all of the following apply when determining the numbers except:
Group of answer choices
All bacteria in the culture are counted
Statistically only plates with between 30-300 colonies should be counted
It is assumed that each colony arose from division of one or a few cells
All of the answer choices are correct
The medium must contain nutrients used by the bacteria
Solution
The statement "All bacteria in the culture are counted" is not applicable when determining the numbers in the enumeration of bacteria in a culture by a plate count. This is because it is practically impossible to count all bacteria in a culture due to their high numbers. Instead, a sample is taken and the number of bacteria in that sample is estimated based on the number of colonies that grow on a plate. This number is then used to estimate the total number of bacteria in the culture.
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