In hybridoma technology, which one of the following enzymes is absent in themyeloma cells that are used for monoclonal antibody production?(A) Hypoxanthine-guanine phosphoribosyltransferase(B) Alanine aminotransferase(C) Triose phosphate isomerase(D) Glycosyltransferase
Question
In hybridoma technology, which one of the following enzymes is absent in themyeloma cells that are used for monoclonal antibody production?(A) Hypoxanthine-guanine phosphoribosyltransferase(B) Alanine aminotransferase(C) Triose phosphate isomerase(D) Glycosyltransferase
Solution
The enzyme that is absent in the myeloma cells used for monoclonal antibody production in hybridoma technology is (A) Hypoxanthine-guanine phosphoribosyltransferase. This enzyme is crucial for the salvage pathway of nucleotide synthesis. Myeloma cells used in hybridoma technology are often HGPRT negative (Hypoxanthine-guanine phosphoribosyltransferase negative) which makes them sensitive to the drug aminopterin, a key component in the HAT medium used during the selection process. This allows only the hybrid cells (hybridoma) to survive, which are HGPRT positive, as they can be produced by fusion of an antibody-producing B cell (which is HGPRT positive) with a myeloma cell (which is HGPRT negative).
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