A western blot is performed on a sample of cells. Which experimental question can be answered with this technique? Group of answer choicesWhat is the sequence of this protein?Is the gene of interest being transcribed in these cells?Is the gene of interest being translated?Is the gene of interest present in the genome?
Question
A western blot is performed on a sample of cells. Which experimental question can be answered with this technique? Group of answer choicesWhat is the sequence of this protein?Is the gene of interest being transcribed in these cells?Is the gene of interest being translated?Is the gene of interest present in the genome?
Solution
The Western blot technique can answer the question: "Is the gene of interest being translated?" This method is used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate proteins by their length, and then transfers them to a membrane where they can be detected using antibodies specific to the target protein. Therefore, it can tell you whether a specific protein (the product of gene translation) is present in the sample.
Similar Questions
What is the purpose of the Western blot technique?To separate proteinsTo sequence DNA fragmentsTo amplify DNA sequencesTo detect specific proteins in a sample
The Southern Blot technique detects A. DNA. B. RNA. C. proteins. D. recombinant DNA. E. specific genetic marker sequence on genes
When performing a Western blot, after running an SDS-PAGE gel, a transfer step is performed.The purpose of this transfer is to:Group of answer choicesAllow the proteins to be visualisedReduce the background ‘noise’ and reduce false positive resultsImmobilise proteins onto a nitrocellulose membraneSeparate the proteins based on their size and charge
Which of the following statements about the generation of B cell receptor (BCR) diversity is FALSE?Select one alternative:Rearrangement of the gene segments coding the variable region of light and heavy chains is the only process by which BCR diversity is generated.Animals have multiple copies of the gene segments that code for the variable region of the heavy and light chains.Rearrangement of the gene segments that code for the variable region of light and heavy chains is a random process.The variable region of the heavy chain is coded by the V, D and J gene segments.
If an individual has a genotype IAi, what protein(s) do their cells make?Group of answer choicesthe version of the enzyme that puts type B carbohydrate molecules on the surface of red blood cellsthe version of the enzyme that puts type A carbohydrate molecules on the surface of red blood cellsthe inactive protein that doesn’t put either type A or type B carbohydrate molecules on the surface of red blood cellsboth the version of the enzyme that puts type A carbohydrate molecules on the surface of red blood cells and the inactive protein
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