How do researchers prepare an intron-free copy of a eukaryotic gene for use in creating transgenic bacteria?Multiple choice question.use taq polymerase to generate thousands of copies of the gene of interestestablish a clone library then expose clones to a probe that contains the DNA of interestuse reverse transcriptase to make cDNA from mature mRNAuse restriction enzymes to cut up both source DNA and vector DNA

The correct answer is: use reverse transcriptase to make cDNA from mature mRNA.

Here is the step-by-step process:

1. Isolate the mature mRNA of the gene of interest from the eukaryotic cell. Mature mRNA is already intron-free because introns are spliced out during RNA processing in eukaryotes.

2. Use reverse transcriptase to synthesize a complementary DNA (cDNA) strand from the mRNA template. Reverse transcriptase is an enzyme that can synthesize DNA from an RNA template, a process that is the reverse of the usual transcription process.

3. Use DNA polymerase to synthesize the second DNA strand, using the cDNA strand as a template. This results in a double-stranded, intron-free DNA copy of the gene.

4. This cDNA can then be inserted into a bacterial plasmid vector and introduced into bacteria to create transgenic bacteria.