Knowee
Questions
Features
Study Tools

20. During gel electrophoresis, separated DNA fragments can be seen as bright orange coloured bands when they are0/4a. Stained with ethidium bromide and exposed to IR radiation.b. Stained with methylene blue and exposed to UV radiation.c. Stained with methylene blue and exposed to IR radiation.d. Stained with ethidium bromide and exposed to UV radiation. 21. After gel electrophoresis, DNA bands are cut out from agarose gel. This is called0/4a. Elutionb. Transformation c. Denaturationd. AnnealingCorrect answera. Elution 22. A small piece of DNA molecule that can carry a foreign DNA segment and replicate inside the host cells is known as0/4a. Marker geneb. Primerc. Cloning vectord. Ori 23. Autonomously replicating circular extra-chromosomal DNA of bacteria is called0/4a. Marker geneb. Plasmidsc. Cosmidsd. BAC 24. Which of the following cloning vectors has very high copy numbers of their genome?0/4a. Plasmidsb. YACc. Bacteriophagesd. BAC 25. The function of a selectable marker is0/4a. Eliminating tarnsformants and permitting non-transformantsb. Elimination of non-transformants and permitting transformantsc. Identify ori sited. To destroy recognition sites 26. Select the wrong statement.0/4a. Transformation is a procedure through which a piece of DNA is introduced in a host bacterium.b. Selectable markers of E. coli include the genes encoding resistance to antibiotics like ampicillin, chloramphenicol, tetracycline, etc.c. Normal E. coli cells have resistance against ampicillin, chloramphenicol etc.d. To link the alien DNA, the vector needs a single or very few recognition sites for restriction enzymes.

Question

  1. During gel electrophoresis, separated DNA fragments can be seen as bright orange coloured bands when they are0/4a. Stained with ethidium bromide and exposed to IR radiation.b. Stained with methylene blue and exposed to UV radiation.c. Stained with methylene blue and exposed to IR radiation.d. Stained with ethidium bromide and exposed to UV radiation. 21. After gel electrophoresis, DNA bands are cut out from agarose gel. This is called0/4a. Elutionb. Transformation c. Denaturationd. AnnealingCorrect answera. Elution 22. A small piece of DNA molecule that can carry a foreign DNA segment and replicate inside the host cells is known as0/4a. Marker geneb. Primerc. Cloning vectord. Ori 23. Autonomously replicating circular extra-chromosomal DNA of bacteria is called0/4a. Marker geneb. Plasmidsc. Cosmidsd. BAC 24. Which of the following cloning vectors has very high copy numbers of their genome?0/4a. Plasmidsb. YACc. Bacteriophagesd. BAC 25. The function of a selectable marker is0/4a. Eliminating tarnsformants and permitting non-transformantsb. Elimination of non-transformants and permitting transformantsc. Identify ori sited. To destroy recognition sites 26. Select the wrong statement.0/4a. Transformation is a procedure through which a piece of DNA is introduced in a host bacterium.b. Selectable markers of E. coli include the genes encoding resistance to antibiotics like ampicillin, chloramphenicol, tetracycline, etc.c. Normal E. coli cells have resistance against ampicillin, chloramphenicol etc.d. To link the alien DNA, the vector needs a single or very few recognition sites for restriction enzymes.
...expand
🧐 Not the exact question you are looking for?Go ask a question

Solution

  1. The correct answer is d. Stained with ethidium bromide and exposed to UV radiation. Ethidium bromide is a fluorescent dye that binds to DNA and fluoresces under UV light, making the DNA visible.

  2. The correct answer is a. Elution. After gel electrophoresis, the DNA bands are cut out from the agarose gel in a process called elution.

  3. The correct answer is c. Cloning vector. A cloning vector is a small piece of DNA, taken from a virus, a plasmid, or the cell of a higher organism, that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes.

  4. The correct answer is b. Plasmids. Plasmids are autonomously replicating circular extra-chromosomal DNA of bacteria.

  5. The correct answer is a. Plasmids. Plasmids have very high copy numbers of their genome.

  6. The correct answer is b. Elimination of non-transformants and permitting transformants. The function of a selectable marker is to identify and select cells that have been successfully transformed with the plasmid vector.

  7. The correct answer is c. Normal E. coli cells have resistance against ampicillin, chloramphenicol etc. This statement is incorrect. Normal E. coli cells do not have resistance against these antibiotics. The resistance is provided by the plasmid vector.

This problem has been solved

Similar Questions

During gel electrophoresis, the DNA sample is mixed with the tracking dye, in order to Choose one answer. Visualize DNA bands at the end All of the above To make the sample loading easy To reduce the carcinogenic nature of the material being used

What is the purpose of using a stain that binds to DNA and fluoresces under UV light? It assists with loading DNA fragments into wells It gives the DNA a negative charge so it will travel through the gel It gives the DNA a positive charge so it will travel through the gel It allows us to visualise where DNA fragments are positioned along the gel

The results of gel electrophoresis are shown below, with four different strands of DNA labeled.Which strand of DNA is the shortest?A.2B.4C.3D.1

Fragments of DNA formed after treatment with endonucleases are separated by the technique of _______a) polymerase chain reactionb) gene amplificationc) blottingd) gel electrophoresis

What should your extracted DNA look like?

1/2

Upgrade your grade with Knowee

Get personalized homework help. Review tough concepts in more detail, or go deeper into your topic by exploring other relevant questions.