The polymerase chain reaction needs the following A. Two primers must flank the sequence of interest on opposite strands B. One primer on the template strand C. Two primers on the same strand D. The two primers must be orientated with 5' ends facing each other
Question
The polymerase chain reaction needs the following A. Two primers must flank the sequence of interest on opposite strands B. One primer on the template strand C. Two primers on the same strand D. The two primers must be orientated with 5' ends facing each other
Solution
The correct answer is A. Two primers must flank the sequence of interest on opposite strands.
Here's why:
The Polymerase Chain Reaction (PCR) is a technique used to amplify a specific segment of DNA. It requires two primers that bind to the DNA on opposite strands. These primers are short pieces of DNA that are complementary to the sequences on either side of the segment you want to
Similar Questions
Which of the following correctly matches the step of polymerase chain reaction (PCR) with its events?Choose 1 answer:Choose 1 answer:(Choice A) Extension - reaction is cooled and primers bind to complementary sequences on template DNA.AExtension - reaction is cooled and primers bind to complementary sequences on template DNA.(Choice B) Annealing - Taq polymerase extends the primers, synthesizing DNABAnnealing - Taq polymerase extends the primers, synthesizing DNA(Choice C, Checked) Denaturation - high temperature separates the DNACDenaturation - high temperature separates the DNA
A DNA template contains the following bases: 5’-ATT GCG TAA-3’ Which of the following sequences represent the complementary strand that would be synthesised by DNA polymerase? Question 4Answer a. 5’-AAT GCG TTA-3’ b. 5’-TTA CGC AAT-3’ c. 5’-ATT GCG TAA-3’ d. 5’-TAA CGC ATT-3’
DNA polymerase synthesis is in ----- direction only A. 3' to 5' B. 5' to 3' C. C to N D. N to C
A. DNA polymerase B. RNA polymerase C. Topoisomerase D. Helicase E. DNA ligase 12.____ catalyzes DNA synthesis in a 5' to 3' direction, adding dNTPs to the 3' end. 13.____which replication enzyme unwinds and separates the DNA strands? 14____ releases tension in supercoils of DNA 15.____ Fills gaps between Okazaki fragments in lagging strand 16.____ Joins gaps in DNA
DNA Polymerase IIIGroup of answer choicesSynthesizes primerRemoves primerProofreads new DNAJoins Okasaki fragmentsUnzips the DNA helixNext
Upgrade your grade with Knowee
Get personalized homework help. Review tough concepts in more detail, or go deeper into your topic by exploring other relevant questions.