Which of the following scenarios would not lead to active mitosis exit? Active Cdc15 Active Dbf2-Mob1 complex Sequestration of Cdc14 by Net1 Inactive Bub2-Bfa1 complex

The scenario that would not lead to active mitosis exit is the "Sequestration of Cdc14 by Net1".

Here's why:

1. Active Cdc15: Cdc15 is a protein kinase that plays a crucial role in the exit of mitosis. When it is active, it promotes the release of Cdc14, a phosphatase that dephosphorylates key substrates allowing exit from mitosis.

2. Active Dbf2-Mob1 complex: This complex is also a protein kinase that is essential for exit from mitosis. It is activated by Cdc15 and contributes to the activation of Cdc14.

3. Sequestration of Cdc14 by Net1: This is the scenario that would not lead to active mitosis exit. Net1 sequesters Cdc14 in the nucleolus, preventing it from dephosphorylating its substrates. This keeps the cell in the mitotic state. Only when Net1 is phosphorylated and inactivated can Cdc14 be released

The scenario that would not lead to active mitosis exit is the "Sequestration of Cdc14 by Net1".

Here's why:

1. Active Cdc15: Cdc15 is a protein kinase that plays a crucial role in the exit of mitosis. When it is active, it promotes the exit from mitosis.

2. Active Dbf2-Mob1 complex: This complex is also a protein kinase that is essential for the exit from mitosis. When it is active, it promotes the exit from mitosis.

3. Sequestration of Cdc14 by Net1: Cdc14 is a protein phosphatase that is required for the exit from mitosis. However, when it is sequestered by Net1, it is kept in the nucleolus and is not available to promote the exit from mitosis. Therefore, this scenario would not lead to active mitosis exit.

4. Inactive Bub2-Bfa1 complex: This complex is a checkpoint that prevents the exit from mitosis until all chromosomes are properly attached to the spindle. When it is inactive, it allows the exit from mitosis.