The dye acriflavine when dissolved in water has its maximum light absorption at 4530 Å andhas maximum florescence emission at 5080 Å. The number of florescence quanta is about 53% ofthe number of quanta absorbed. What percentage of absorbed light energy is emitted asflorescence?
Question
The dye acriflavine when dissolved in water has its maximum light absorption at 4530 Å andhas maximum florescence emission at 5080 Å. The number of florescence quanta is about 53% ofthe number of quanta absorbed. What percentage of absorbed light energy is emitted asflorescence?
Solution
The energy of a photon is given by the equation E = hc/λ, where h is Planck's constant, c is the speed of light, and λ is the wavelength of the light.
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First, we need to calculate the energy of the absorbed light. The wavelength of the absorbed light is given as 4530 Å. Convert this to meters: 4530 Å = 4530 x 10^-10 m.
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Plug these values into the equation to find the energy of the absorbed light: E(absorbed) = (6.626 x 10^-34 J*s)(3.00 x 10^8 m/s) / (4530 x 10^-10 m) = 4.39 x 10^-19 J.
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Next, calculate the energy of the emitted light in the same way. The wavelength of the emitted light is given as 5080 Å = 5080 x 10^-10 m.
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Plug these values into the equation to find the energy of the emitted light: E(emitted) = (6.626 x 10^-34 J*s)(3.00 x 10^8 m/s) / (5080 x 10^-10 m) = 3.91 x 10^-19 J.
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The problem states that the number of fluorescence quanta is about 53% of the number of quanta absorbed. This means that the total energy emitted is 53% of the energy calculated in step 4: E(total emitted) = 0.53 * 3.91 x 10^-19 J = 2.07 x 10^-19 J.
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Finally, calculate the percentage of absorbed light energy that is emitted as fluorescence. This is given by the ratio of the total energy emitted to the energy absorbed, multiplied by 100%: (2.07 x 10^-19 J / 4.39 x 10^-19 J) * 100% = 47.15%.
So, about 47.15% of the absorbed light energy is emitted as fluorescence.
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