A new amino acid has been identified with the following pka values:pKa (-COOH) = 2.32pKa (-NH3+) = 9.68What is the pI?
Question
A new amino acid has been identified with the following pka values:pKa (-COOH) = 2.32pKa (-NH3+) = 9.68What is the pI?
Solution
The isoelectric point (pI) of an amino acid is the pH at which the amino acid has a net charge of zero. It can be calculated using the pKa values of the amino acid.
For an amino acid with two ionizable groups (like the one in the question), the pI can be calculated as the average of the two pKa values.
So, in this case, the pI would be calculated as follows:
pI = (pKa1 + pKa2) / 2 pI = (2.32 + 9.68) / 2 pI = 12 / 2 pI = 6
So, the pI of the new amino acid is 6.
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