2H 2 O 2 (l)→ 2H 2 O (l)+ O 2 This reaction can be catalyzed by an enzyme (catalase) or by a non-biological catalyst (Manganese IV oxide)Method:Put 3 cm3 of hydrogen peroxide in a test tube.Add fresh potato strips and shake gently.Keep your thumb on top of the test tube, or use a stopper, to retain the gas.Do the “glowing splint” test → the splint relightsPositive control: repeat original experiment using manganese IV oxide → bubbles of O2 formConclusion: Reaction happens because of a catalyst1st negative control: repeat original experiment using boiled potato strips → nothing happensConclusion: Enzymes denature when they are at high temperatures2nd negative control: repeat original experiment using water instead of hydrogen peroxide → nothing happensConclusion: hydrogen peroxide is the substrate3rd negative control: repeat in a cold environment, the effervescence should be slowerConclusion: enzymes don’t work as well in the cold
Question
2H 2 O 2 (l)→ 2H 2 O (l)+ O 2 This reaction can be catalyzed by an enzyme (catalase) or by a non-biological catalyst (Manganese IV oxide)Method:Put 3 cm3 of hydrogen peroxide in a test tube.Add fresh potato strips and shake gently.Keep your thumb on top of the test tube, or use a stopper, to retain the gas.Do the “glowing splint” test → the splint relightsPositive control: repeat original experiment using manganese IV oxide → bubbles of O2 formConclusion: Reaction happens because of a catalyst1st negative control: repeat original experiment using boiled potato strips → nothing happensConclusion: Enzymes denature when they are at high temperatures2nd negative control: repeat original experiment using water instead of hydrogen peroxide → nothing happensConclusion: hydrogen peroxide is the substrate3rd negative control: repeat in a cold environment, the effervescence should be slowerConclusion: enzymes don’t work as well in the cold
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