Select the most appropriate reason for why clear areas have appeared around the paper discs.These are areas where the bacteria have killed each other.These are areas where the agar has not set properly.These are areas where the antimicrobial has killed the bacteria or stopped them growing.These are areas of agar that were not inoculated with bacteria.

A sterile agar plate, I, is streaked with a pure culture of bacteria by means of aseptic techniques. Paper discs treated with the antibiotics Aureomycin (A) and penicillin (P) are placed at opposite sides of the plate, as shown in the diagram above. The plate is examined after a 24-hour incubation period, and a clear ring is discovered around disc A, but not around disc P. Within the clear ring around disc A, a single bacterial colony with physical characteristics like those of the pure culture is observed. A second sterile agar plate, II, is then streaked with this single colony and also incubated with antibiotics.

A clear, circular area of media around a disk containing an antimicrobial agent, where bacteria are unable to grow due to the presence of a drug that impedes their growth. ____.A double zone of inhibitionA zone of resistanceA zone of inhibition

Explain how each scenario listed below would affect the Kirby Bauer disc diffusion test results:i) Thicker agar used (2 marks)

Bacterial smears are fixed before staining toGroup of answer choicesmake the cells visible.accept stain.affix the cells to the slide.make their walls permeable.

Which of the following is a description of the elevation of a bacterial colony above the surface of an agar plate? Group of answer choices Filamentous Irregular Round Convex Rhizoid