In reversed phase chromatography, a non-polar stationary phase is used, non-polar analytes are retained more strongly, and a more non-polar eluent will cause shorter retention times for analytes in a chromatographic analysis.Group of answer choicesTrueFalse
Question
In reversed phase chromatography, a non-polar stationary phase is used, non-polar analytes are retained more strongly, and a more non-polar eluent will cause shorter retention times for analytes in a chromatographic analysis.Group of answer choicesTrueFalse
Solution
True - In reversed phase chromatography, a non-polar stationary phase is indeed used. Non-polar analytes interact more strongly with the non-polar stationary phase and thus are retained longer. Using a more non-polar eluent (mobile phase) will decrease the polarity difference between the stationary phase and the mobile phase, causing non-polar analytes to elute faster, thus resulting in shorter retention times.
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