Gold standard for detection of S equi*1 pointColumbia CNA blood agarDirect SmearBacterial isolation with chocolate agarH & E stainNone of the above
Question
Gold standard for detection of S equi*1 pointColumbia CNA blood agarDirect SmearBacterial isolation with chocolate agarH & E stainNone of the above
Solution
The gold standard for detection of Streptococcus equi, often abbreviated as S. equi, is bacterial isolation. However, the medium used is not chocolate agar but rather a selective medium such as Columbia CNA blood agar.
Here's a step-by-step breakdown:
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Sample Collection: The first step in detecting S. equi is to collect a sample. This is typically done by swabbing the nasopharynx or by aspirating pus from abscesses.
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Plating on Selective Medium: The sample is then plated on a selective medium, such as Columbia CNA blood agar, which encourages the growth of gram-positive bacteria like S. equi and inhibits the growth of gram-negative bacteria.
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Incubation: The plate is incubated at a suitable temperature (usually 37 degrees Celsius) for 24-48 hours to allow the bacteria to grow.
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Identification: After incubation, the colonies of bacteria are identified based on their morphology, hemolytic properties, and other biochemical tests.
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Confirmation: The identification of S. equi can be confirmed by further tests such as PCR or serological tests.
So, the correct answer to your question would be "Columbia CNA blood agar".
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Nucleic acid detection techniques:ANSWERare less sensitive than the antigen–antibody-based tests.are used to culture animal viruses in the lab.are much slower than the antigen–antibody-based tests.can detect new viruses and early-stage infections that antibody–antigen tests are likely to miss.
is branch of immunology that traditionally deals with in vitro diagnostic testing of serum. The ability to detect even minute amounts of target antibodies or antigens, , is an important characteristic of such in vitro tests, as is , or the test’s ability to eliminate reactions with unrelated or distantly related antigens. tests, such as the use of a latex test for strep throat, are an example of accurate in vitro serological tests, which produce antibody-mediated cell clumping that can be visualized for positive identification of microbial infection. In contrast, reactions, such as serotyping for species identification, produce insoluble antibody-antigen complexes in vitro that can be visualized for positive identification of a microbial infection.
Nucleic acid detection techniques:ANSWERare less sensitive than the antigen–antibody-based tests.can detect new viruses and early-stage infections that antibody–antigen tests are likely to miss.are much slower than the antigen–antibody-based tests.are used to culture animal viruses in the lab.
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