If the nontemplate strand of a gene in E. coli had the sequence: 5 ′ -TTGACA-(18 bases)-TATAAT-(8 bases)-GCCTTCCAGTG-3 ′ what nucleotide sequence would be present in the RNA transcript of this gene?
Question
If the nontemplate strand of a gene in E. coli had the sequence: 5 ′ -TTGACA-(18 bases)-TATAAT-(8 bases)-GCCTTCCAGTG-3 ′ what nucleotide sequence would be present in the RNA transcript of this gene?
Solution
The RNA transcript is synthesized from the template strand of DNA, which is the complementary strand to the non-template strand. The non-template strand is also known as the coding strand because its sequence matches the RNA transcript (except that T in DNA is replaced by U in RNA).
Given the non-template strand of DNA: 5′ -TTGACA-(18 bases)-TATAAT-(8 bases)-GCCTTCCAGTG-3′
The RNA transcript would have the same sequence, but with uracil (U) instead of thymine (T). Therefore, the sequence of the RNA transcript would be:
5′ -UUGACA-(18 bases)-UAUAAU-(8 bases)-GCCUUCCAGUG-3′
Please note that the actual sequence of the 18 bases and 8 bases in between the given sequences would also be transcribed into RNA, but since they are not provided, they are not included in the RNA sequence.
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