State the purpose of the separator and suppressor in suppressed-ion chromatography. For cation chromatography, why is the suppressor an anion-exchange membrane?
Question
State the purpose of the separator and suppressor in suppressed-ion chromatography. For cation chromatography, why is the suppressor an anion-exchange membrane?
Solution
In suppressed-ion chromatography, the separator and suppressor serve distinct purposes.
-
Separator: The separator column's purpose is to separate the ions in the sample based on their interaction with the stationary phase of the column. This interaction is influenced by the charge, size, and polarity of the ions. The ions are then eluted at different times, which allows for their identification and quantification.
-
Suppressor: The suppressor's role is to reduce the background conductivity of the eluent (mobile phase) while allowing the separated ions from the sample to pass through. This increases the sensitivity of the detection, as the signal-to-noise ratio is improved.
In cation chromatography, the suppressor is an anion-exchange membrane because it needs to exchange the anions in the eluent with hydrogen ions (H+). This process converts the eluent into a weak acid, which has lower conductivity than the original eluent. This allows the cations from the sample, which have been separated in the separator column, to stand out more clearly in the detection process.
Similar Questions
Outline briefly the ion-exchange processes taking place in the suppressor column of an anion chromatograph if the eluate contains KOH and KNO3 (NO3- is the analyte anion and KOH is the eluent). How will these ion-exchange processes affect sensitivity? Write the stoichiometric equation for the ion-exchange reaction if the suppressor column is packed with sulfonic resin beads.
The ion-exchange chromatography is widely used for protein separation(a) Describe the principles of ion-exchange chromatography;(b) Explain why changing the pH allows different amino acids to elute from the column at differenttimes
What is the separation principles that govern size exclusion chromatography?
The gas-solid chromatography is ______________ chromatography as per basic principle involved.a.Adsorptionb.Absorptionc.Exclusiond.Ion-exchange
The correct statement among the following, for a "chromatography" purification method is :JEE Main 2024 (06 Apr Shift 2)AOrganic compounds run faster than solvent in the thin layer chromatographic plate.BRfR𝑓 is an integral value.CRfR𝑓 of a polar compound is smaller than that of a non-polar compound.DNon-polar compounds are retained at top and polar compounds come down in column chromato
Upgrade your grade with Knowee
Get personalized homework help. Review tough concepts in more detail, or go deeper into your topic by exploring other relevant questions.