Source and vector DNA that is cut with the same restriction enzyme can have complementary ______ that can base pair with each other and be sealed by ligase.Multiple choice question.primerspromotersintron sitessticky ends
Question
Source and vector DNA that is cut with the same restriction enzyme can have complementary ______ that can base pair with each other and be sealed by ligase.Multiple choice question.primerspromotersintron sitessticky ends
Solution
The correct answer is "sticky ends".
Step by step explanation:
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The process begins with the cutting of both source and vector DNA with the same restriction enzyme.
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Restriction enzymes are proteins that recognize specific sequences in DNA and cut at those sites.
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When the restriction enzyme cuts the DNA, it can leave either "blunt ends" or "sticky ends".
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"Sticky ends" are overhangs of single-stranded DNA at the site of the cut.
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Because the source and vector DNA were cut with the same restriction enzyme, their sticky ends are complementary.
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These complementary sticky ends can base pair with each other, meaning they can form hydrogen bonds and stick together.
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Finally, the enzyme DNA ligase seals the gaps in the sugar-phosphate backbone of the DNA, making the connection between the source and vector DNA permanent.
So, the correct answer to the question "Source and vector DNA that is cut with the same restriction enzyme can have complementary ______ that can base pair with each other and be sealed by ligase" is "sticky ends".
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