Which direct immunologic method can be used to determine the presence of a specific microbe in a specimen?Multiple choice question.Acid-fast stainKirby-Bauer testPolymerase chain reactionDirect fluorescence antigen testGram stain

Sample QuestionPlease choose all methods used to identify bacteria.Microscopic appearance

Which of the following uses a known fluorescent antibody to detect the presence of a target antigen?Multiple choice question.Direct antigen testRadioimmunoassayIndirect immunofluorescence testDirect immunofluorescence testDirect ELISACapture ELISA

Fill in the following blanks.a. 1 μm = mb. 1 = 10-9 mc. 1 μm = nm2. Which type of microscope would be best to use to observe each ofthe following?a. a stained bacterial smearb. unstained bacterial cells: the cells are small, and no detail is neededc. unstained live tissue when it is desirable to see someintracellular detaild. a sample that emits light when illuminated with ultraviolet lighte. intracellular detail of a cell that is 1 μm longf. unstained live cells in which intracellular structures are shownin color

is branch of immunology that traditionally deals with in vitro diagnostic testing of serum. The ability to detect even minute amounts of target antibodies or antigens, , is an important characteristic of such in vitro tests, as is , or the test’s ability to eliminate reactions with unrelated or distantly related antigens. tests, such as the use of a latex test for strep throat, are an example of accurate in vitro serological tests, which produce antibody-mediated cell clumping that can be visualized for positive identification of microbial infection. In contrast, reactions, such as serotyping for species identification, produce insoluble antibody-antigen complexes in vitro that can be visualized for positive identification of a microbial infection.

Direct immunofluorescent antibody testing is especially useful in identifying which type of bacteria?Multiple choice question.Most pathogenic cocciMost pathogenic bacilliSpirillumCertain pathogenic spirochetes