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How would you make a 10x sample dilution to a total volume of 1 ml?

Question

How would you make a 10x sample dilution to a total volume of 1 ml?

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Solution

To make a 10x sample dilution to a total volume of 1 ml, follow these steps:

  1. First, determine the volume of the sample you need. Since you're making a 10x dilution, you'll need 1/10 of the final volume. In this case, that's 1 ml / 10 = 0.1 ml of your sample.

  2. Next, calculate the volume of the diluent (the substance you're diluting your sample in, often distilled water or a buffer solution) you need. This is the final volume minus the sample volume. So, 1 ml - 0.1 ml = 0.9 ml.

  3. Now, take your sample and add it to a new container.

  4. Then, add your diluent to the same container until you reach the final volume of 1 ml. In this case, you'll be adding 0.9 ml of diluent.

  5. Finally, mix the solution thoroughly to ensure the sample is evenly distributed throughout.

Remember to use clean, sterile equipment to avoid contamination, and to handle all samples and reagents with care.

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