According to the small size of the particle, which type of chromatographic separation is applicable?Question 4AnswerA.High- performance liquid chromatography (HPLC)B.Gel chromatographyC.Fast protein liquid chromatography (FPLC)D.Paper chromatography

What is the separation principles that govern size exclusion chromatography?

What is chromatography used to separate?An insoluble solid from a liquidTwo or more liquids with different boiling pointsDifferent substances dissolved in a liquidTwo or more different gases2Which of the following statements about paper chromatography is not true?Less soluble substances travel further up the paperSubstances are separated based on how attracted they are to each phaseThe stationary phase is the paperThe mobile phase is the solvent3What are Rf values useful for?To determine how much solvent to use in the chromatography procedureTo calculate how much paper to useTo identify unknown samples by comparing them with known samplesTo determine the number of different substances in a solvent4How is the Rf value of a substance calculated?Distance moved by substance divided by distance moved by solventDistance moved by solvent minus distance moved by substanceDistance moved by solvent divided by distance moved by substanceDistance moved by substance multiplied by distance moved by solvent5In the core practical ‘Paper chromatography to investigate the composition of ink’, a line is drawn at the bottom of the paper onto which spots of known ink colourings plus an unknown sample are placed. There are several key points to remember when setting up this practical. Which of the following is incorrect? The line must be above the water levelThe line must be in penThe sides of the paper must not touch the beakerThe sample spots should not spread out too far6On a paper chromatogram only one spot is produced. What does this tell you? The paper was not large enoughThe substance being separated is an impure substanceToo much solvent was usedThe substance being separated is a pure substance

What is the principle of the paper chromatography ?a.Partitionb.Adsorptionc.A and Bd.None of the above

Consider gel-filtration chromatograpy and select the correct statement(s).Question 3Answer1.The larger the volume a particle wanders in, the earlier it elutes.2.The total column volume equals to the sum of the internal bead volume and of the volume between the beads.3.The larger the volume a particle wanders in, the later it elutes.4.The solid phase is composed of porous beads.5.It separates the analyte components according to their size

In which type of chromatography the stationary phase held in a narrow tubea.Liquid Chromatographyb.Paper chromatographyc.column Chromatographyd.Thin layer chromatographyClear my choice