Which of the following technique(s) is/are involved in recombinant DNA technology? Cleavage of DNA at specific sites by restriction nucleases DNA ligation DNA cloning Nucleic acid hybridization All of the above
Question
Which of the following technique(s) is/are involved in recombinant DNA technology? Cleavage of DNA at specific sites by restriction nucleases DNA ligation DNA cloning Nucleic acid hybridization All of the above
Solution
All of the above techniques are involved in recombinant DNA technology.
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Cleavage of DNA at specific sites by restriction nucleases: This is the first step in recombinant DNA technology. Restriction enzymes (also known as restriction nucleases) recognize specific sequences in the DNA and cut it at those sites.
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DNA ligation: After the DNA has been cut, the desired gene is inserted and then the DNA is sealed back together by an enzyme called DNA ligase. This process is known as ligation.
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DNA cloning: Once the desired gene has been inserted into the DNA, it needs to be replicated to produce many copies. This is done by inserting the recombinant DNA into a host organism (like bacteria) which will replicate the DNA along with its own. This process is known as cloning.
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Nucleic acid hybridization: This is a technique used to identify and locate specific sequences of DNA. It involves creating a complementary sequence (probe) that will bind to the desired sequence in the DNA. This probe is often labeled with a radioactive or fluorescent tag so it can be easily detected.
So, all of the above techniques are involved in recombinant DNA technology.
Similar Questions
Which of the following procedures is different from a usual step in a recombinant DNA experiment?Group of answer choicesrecombining pieces of DNA from different organismsinducing a mutation in a source chromosomerecombining pieces of DNA from different organismscutting DNA with a restriction enzyme
What is recombinant DNA technology?
Which of the following terms does not involve recombinant DNA (genetic manipulation) technologies?Group of answer choicesnone of the given choices involves such technologiestesting the levels of salt tolerance of different linesbreeding of different lines to created new highly tolerant linesamplifying salt-tolerance related genes
Recombinant DNA is genetic materialMultiple choice question.spliced together from multiple sources.that contains STRs for DNA profiling.that has undergone 10 or more mutations.that is no longer expressed.
What is Recombinant DNA Technology? Techniques used to regulate energy intake and output The ability to recombine and manipulate metabolic output of cells Technology used to examine the phenotype of a recombinant organisms The ability to manipulate and recombine fragments of DNA in a test tube or in an organism The ability to detect molecules at the nano scale.
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