Knowee
Questions
Features
Study Tools

DNA fragments separate on an electrophoresis gel with the A) largest segments traveling furthest. B) smallest segments traveling furthest. C) most negatively charged pieces traveling quickest. D) most positively charged pieces moving backward

Question

DNA fragments separate on an electrophoresis gel with the A) largest segments traveling furthest. B) smallest segments traveling furthest. C) most negatively charged pieces traveling quickest. D) most positively charged pieces moving backward

🧐 Not the exact question you are looking for?Go ask a question

Solution

The correct answer is B) smallest segments traveling furthest.

Here's a step-by-step explanation:

  1. DNA electrophoresis is a method used to sort DNA molecules by size.

  2. The DNA fragments are placed in a gel, and an electric current is applied.

  3. DNA molecules are negatively charged, so when an electric current is applied, they move towards the positive electrode.

  4. The gel acts like a sieve, smaller pieces of DNA move through the gel more easily than larger pieces.

  5. Therefore, the smallest segments of DNA will travel the furthest during electrophoresis.

So, the size of the DNA fragment determines how far it travels, not its charge. The charge on all DNA molecules is the same because it's determined by the phosphate backbone, which is the same for all DNA molecules.

This problem has been solved

Similar Questions

Which of the following statements is correct regarding agarose gel electrophoresis? Question 3Answer a. A small DNA fragment will travel faster than a large fragment in a direction based on the fragment's negative charge. b. A small DNA fragment will travel faster than a large fragment in a direction based on the fragment's positive charge. c. A small DNA fragment will travel slower than a large fragment in a direction based on the fragment's positive charge. d. A small DNA fragment will travel slower than a large fragment in a direction based on the fragment's negative charge.

Gel electrophoresis separates fragments of DNA according to what property? Length in nucleotides Organism they originate from Protein they code for2. QuestionWhat is the overall charge on DNA? Positive charge Negative charge DNA has no charge3. QuestionReferring to the image below, on which side of the gel tank should the positive electrode be placed so that DNA in the well migrates in the direction of the arrow? Left side Right side4. QuestionTwo fragments of DNA are loaded into a well on a gel. One fragment is 50 nucleotides in length, the other fragment is 200 nucleotides in length. A current is applied to the gel for 10 minutes. Which fragment will migrate further through the gel? The fragment that is 50 nucleotides in length The fragment that is 200 nucleotides in length Both fragments will travel the same distance5. QuestionWhat is the purpose of using a stain that binds to DNA and fluoresces under UV light? It assists with loading DNA fragments into wells It gives the DNA a negative charge so it will travel through the gel It gives the DNA a positive charge so it will travel through the gel It allows us to visualise where DNA fragments are positioned along the gel6. QuestionThe image below shows the results of a gel run. Based on these results, which of the following statements is correct? The DNA fragments in band A are shorter than the DNA fragments in band C The DNA fragments in band A are longer than the DNA fragments in band C The DNA fragments in band A are roughly the same length as the DNA fragments in band C7. QuestionBased on the gel results in the previous question, which of the following statements is correct? The DNA fragments in band B are shorter than the DNA fragments in band C The DNA fragments in band B are longer than the DNA fragments in band C The DNA fragments in band B are roughly the same length as the DNA fragments in band C8. QuestionEach person inherits two copies of a certain gene from their biological parents. There are two versions of the gene that can be inherited, version A and version B. Version A is longer than version B.DNA samples are collected from three patients. The gene of interest is copied using PCR and each sample is loaded into wells on a gel. The gel is run and a DNA profile is obtained (as seen in the image below). Which versions of the gene does each patient have? Justify your response.

el electrophoresis separates fragments of DNA according to what property? Length in nucleotides Organism they originate from Protein they code for

4. DNA sequences can be cut into smaller fragments by A. gel electrophoresis B. DNA ligase C. restriction endonucleases D. DNA polymerase

The results of gel electrophoresis are shown below, with four different strands of DNA labeled.Which strand of DNA is the shortest?A.2B.4C.3D.1

1/3

Upgrade your grade with Knowee

Get personalized homework help. Review tough concepts in more detail, or go deeper into your topic by exploring other relevant questions.