PFK-1 catalyses the:You can respond oncedephosphorylation of fructose 1,6-bisphosphate to fructose 6-phosphatecleavage of fructose 1,6-bisphosphate into DHAP and GA-3-P.transfer of phosphate from PEP to ADP to generate pyruvate and ATP.phosphorylation of fructose 6-phosphate to fructose 1,6-bisphosphate.interconversion of glucose 6-phosphate and fructose 6-phosphate.dephosphorylation of fructose 2,6-bisphosphate to fructose 6-phosphatephosphorylation of fructose 6-phosphate to fructose 2,6-bisphosphate

The reaction catalysed by PFK-1 is important because it:You can respond onceis freely reversible and hence the enzyme can be used in glycolysis and gluconeogenesis.is the first committed step of, and the most important control element in, glycolysis.generates two 3-carbon molecules from a 6-carbon molecule, so that the pay-off phase of glycolysis occurs twice.generates two ATP molecules per glucose molecule that enters glycolysis.

PFK1 is a key enzyme in the mammalian glycolytic pathway. Discuss the effects of ATP, AMP, PEP and citrate on PFK1 activity and, therefore, on cellular metabolism more broadly (10 marks) Discuss the effects of fructose 2,6 bisphosphate on PFK1 activity and the significance of this regulation (5marks) Why is this step so heavily controlled? (5 marks)

The phosphorylation of glucose to glucose-6-P in the glycolysis pathway is catalyzed by glucokinase. Which enzyme in the gluconeogenesis pathway catalyzes the reverse reaction (G6P > Glucose)? A. Glukose-6-fosfatase / Glucose-6-phosphatase B. Piruvaatkinase / Pyruvate kinase C. Heksokinase / Hexokinase D. Fosfofruktokinase / Phosphofructokinase E. Fruktose-1,6-bifosfatase / Fructose-1,6-bisphosphatase

During glycolysis, phosphofructokinase converts fructose 6-phosphate (F6P) into fructose 1,6-bisphosphate (F1,6BP), as shown below.  Aldolase then converts F1,6BP into glyceraldehyde 3-phosphate (GAP) and dihydroxyacetone phosphate (DHAP).Respectively, phosphofructokinase and aldolase are examples of:A.an isomerase and a hydrolase.B.a transferase and a lyase.C.a ligase and an isomerase.D.an oxidoreductase and a transferase.

Phosphofructokinase-1 converts Fructose 6-phosphate to Fructose 1,6- bisphosphate. Which statement about the reaction is CORRECT? Under anaerobic conditions, this reaction plays an important role in regenerating NAD+ for use in the “pay-off” phase of glycolysis. This reaction occurs in the cytoplasm and is performed by an enzyme shared between glycolysis and gluconeogenesis. This is a readily reversible reaction which is allosterically regulated by Fructose 1,6- bisphosphate. The bifunctional PFK-2/FBPase-2 enzyme controls the concentration of the allosteric regulator of this reaction.