Which experimental procedure(s) must scientists use to determine Vmax and KM of an enzymatic reaction using the Michaelis-Menten model? They must ensure that:they only measure the initial reaction rate for each substrate concentration.total enzyme concentration is much greater than the KM of the reaction.each initial substrate concentration tested is much greater than enzyme concentration.A.I onlyB.III onlyC.I and II onlyD.I and III onlySubmit
Question
Which experimental procedure(s) must scientists use to determine Vmax and KM of an enzymatic reaction using the Michaelis-Menten model? They must ensure that:they only measure the initial reaction rate for each substrate concentration.total enzyme concentration is much greater than the KM of the reaction.each initial substrate concentration tested is much greater than enzyme concentration.A.I onlyB.III onlyC.I and II onlyD.I and III onlySubmit
Solution
To determine Vmax and KM of an enzymatic reaction using the Michaelis-Menten model, scientists must follow these experimental procedures:
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Measure the initial reaction rate for each substrate concentration: This is important because the initial rate is when the rate of the reaction is at its fastest and is not affected by the depletion of substrate or the accumulation of product.
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Ensure that the total enzyme concentration is much greater than the KM of the reaction: This is not necessary. The Michaelis-Menten model assumes that the substrate concentration ([S]) is much larger than the enzyme concentration ([E]). This is because the model is derived under the assumption that the reaction has reached a steady state, which is only valid when [S] >> [E].
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Each initial substrate concentration tested is much greater than enzyme concentration: This is necessary because the Michaelis-Menten model assumes that the substrate concentration is much larger than the enzyme concentration.
Therefore, the correct answer is D. I and III only.
Similar Questions
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