Homework:Watch videos and prepare for the practical on Monday.Practical - making a wet mount slide and and viewing an onion cell through the microscopeWrite up a mini report for the experimentHeading/titlethe purposeequipmentrisk assessmentmethodWrite up a risk assessment for the practical
Question
Homework:Watch videos and prepare for the practical on Monday.Practical - making a wet mount slide and and viewing an onion cell through the microscopeWrite up a mini report for the experimentHeading/titlethe purposeequipmentrisk assessmentmethodWrite up a risk assessment for the practical
Solution
Title: Observation of Onion Cells Under Microscope
Purpose: The purpose of this practical is to learn how to prepare a wet mount slide and observe the structure of an onion cell under a microscope.
Equipment:
- Microscope
- Onion
- Scalpel
- Tweezers
- Glass slide and cover slip
- Iodine solution
- Dropper
- Tissue paper
Risk Assessment:
- Handling the Scalpel: Care must be taken while using the scalpel to cut the onion. It is sharp and can cause injury if not handled properly.
- Using the Microscope: The microscope is a delicate and expensive piece of equipment. It should be handled with care to avoid damage.
- Iodine Solution: Iodine solution can stain clothes and skin. It should be handled carefully. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Method:
- Cut a small piece of onion using the scalpel.
- Using the tweezers, peel off a thin layer of the onion.
- Place the thin layer on the glass slide.
- Add a drop of iodine solution to the onion layer using the dropper. The iodine solution will help to stain the cells, making them easier to see.
- Carefully place the cover slip over the onion layer.
- Place the slide under the microscope and adjust the focus until the cells are clearly visible.
- Observe and note down the structure of the onion cells.
Risk Assessment for the Practical: This practical involves the use of sharp objects (scalpel), chemicals (iodine solution), and delicate equipment (microscope). Proper safety measures should be taken to avoid any accidents. Always handle the scalpel with care and point it away from yourself and others. When using the iodine solution, avoid contact with skin and eyes. If contact occurs, rinse immediately with water and seek medical advice. Handle the microscope with care to avoid damaging it. Always supervise children during this practical.
Similar Questions
viewing an onion cell through the microscopeWrite up a mini report for the experimentHeading/titlethe purposeequipmentrisk assessmentmethodWrite up a risk assessment for the practical
Give a step-by-step procedure for preparing a wet mount slide to view onion cells under a light microscope.
A student prepared some onion cells. The student viewed the onion cells using a light microscope. This is the method used. 1. Cut an onion into pieces using a sharp knife. 2. Peel off a thin layer of onion epidermis from one piece of onion. 3. Place the onion epidermis onto a microscope slide in a single flat layer. 4. Add three drops of iodine solution. 5. Slowly lower a cover slip at an angle onto the onion epidermis. 6. Place the slide on the stage of the microscope. 4. Page 6 of 16Sugars absorbed via active transport would show a decrease in aborption rate where there is no respiration occuring as it needs energy to carry out active transport and from the table we can see that the sugars glucose and galactose both significantly decreased whichi informs us that those two sugards can be absorbed by active transportBecause every single sugar got absorbed which informs us that some form of diffusionhas taken place(a) The table below shows a risk assessment for this experiment. Complete the table.HazardRiskPlan to minimise risk Iodine solution is an irritant May cause allergic reaction or skin rashSharp knife(2) (b) Give a reason for each of the following steps in the method. A thin layer of onion epidermis is used. ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ Iodine solution is added to the onion epidermis. ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ The cover slip is lowered onto the onion epidermis at an angle. ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ (3) Page 7 of 16Figure 1 shows what the student saw under the microscope at a magnification of ×400. Figure 1 (c) The length of cell Z in Figure 1 is 4.8 cm. Calculate the real length of cell Z. Give your answer in micrometres (μm). ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ Real length of cell Z = _______________μm (5) Page 8 of 16Figure 2 shows the student’s drawing of Figure 1. Figure 2 (d) Give two ways the student could improve the drawing in Figure 2. 1 _________________________________________________________________ ___________________________________________________________________ 2 _________________________________________________________________ ___________________________________________________________________ (2) (e) Onion cells can be seen using an electron microscope. Give two ways onion cells would look different when seen using an electron microscope. 1 _________________________________________________________________ ___________________________________________________________________ 2 _________________________________________________________________ ___________________________________________________________________ (2) (Total 14 marks)
I thought I did well in this sample because I showed examples of what was needed for this experiment and demonstrated why these materials can be a risk hazard. I could’ve improved my work by __________. This helped me in my summative task because it deepened my understanding of how to identify and classify organisms.
Give two tips for the effective use of a microscope.
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