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What are three key proteins required during DNA unwinding at the replication fork?Group of answer choicesDNA helicase, DNA gyrase, DNA polymeraseDNA polymerase, DNA gyrase, DNA ligaseDNA helicase, DNA gyrase, single stranded DNA binding proteinDNA polymerase, DNA gyrase, DNA ligase

Question

What are three key proteins required during DNA unwinding at the replication fork?Group of answer choicesDNA helicase, DNA gyrase, DNA polymeraseDNA polymerase, DNA gyrase, DNA ligaseDNA helicase, DNA gyrase, single stranded DNA binding proteinDNA polymerase, DNA gyrase, DNA ligase

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Solution

The three key proteins required during DNA unwinding at the replication fork are DNA helicase, DNA gyrase, and single stranded DNA binding protein.

Step 1: DNA helicase unwinds the double-stranded DNA by breaking the hydrogen bonds between the base pairs. This creates a replication fork.

Step 2: DNA gyrase relieves the tension in the DNA molecule that is created by the unwinding process. It does this by introducing negative supercoils into the DNA.

Step 3: Single stranded DNA binding proteins bind to the single-stranded DNA to prevent the strands from reannealing and to protect them from degradation.

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The following are enzymes required in DNA replication except A. DNA helicase B. Topoisomerase C. DNA primase D. DNA synthase

A. DNA polymerase B. RNA polymerase C. Topoisomerase D. Helicase E. DNA ligase 12.____ catalyzes DNA synthesis in a 5' to 3' direction, adding dNTPs to the 3' end. 13.____which replication enzyme unwinds and separates the DNA strands? 14____ releases tension in supercoils of DNA 15.____ Fills gaps between Okazaki fragments in lagging strand 16.____ Joins gaps in DNA

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