The DNA polymerase used for a PCR has to be resistant to what? Low temperature High temperature Light Salt concentration Time
Question
The DNA polymerase used for a PCR has to be resistant to what? Low temperature High temperature Light Salt concentration Time
Solution
The DNA polymerase used for a PCR (Polymerase Chain Reaction) needs to be resistant to high temperatures. This is because PCR involves a cycle of heating and cooling to denature the DNA, anneal the primers, and extend the DNA strands. Normal DNA polymerases would denature (lose their function) at the high temperatures used in PCR. Therefore, a special type of DNA polymerase that is resistant to these high temperatures, such as Taq polymerase, is used. Taq polymerase is derived from the thermophilic bacterium Thermus aquaticus, which lives in hot springs and can withstand the high temperatures used in PCR.
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