This work demonstrates that RT-dPCR significantly improves accuracy and reduces the false negative rate of diagnostics of SARS-CoV-2 in pharyngeal swab specimens, which is more convenient and simpler to sampling. Furthermore, dPCR is more sensitive and suitable for low virus load specimens from the patients under isolation and observation who may not be exhibiting clinical symptoms. Finally, RT-dPCR could be used to quantitative monitoring the convalescents to evaluate disease progression. list the conclusions

Existem diferentes metodologias para a detecção de SARS-CoV-2, bem como indicações distintas para cada técnica. Qual é a principal diferença entre as técnicas de RT-PCR e sorológicas? A. O RT-PCR detecta o antígeno do vírus, enquanto as técnicas sorológicas detectam RNA.B. O RT-PCR quantifica os anticorpos do vírus, enquanto as técnicas sorológicas detectam RNA.C. O RT-PCR detecta o material genético do vírus, enquanto as técnicas sorológicas detectam os anticorpos.

Diagnosic tests for COVID-19 include:Multiple select question.immunological tests measuring either antigens or antibodies.culturing SARS-CoV-2 from blood samples.nucleic acid amplification tests such as RT-PCR.Gram staining.

SARS-CoV-2 is the etiological agent of COVID-19. Most of SARS-CoV-2 carriers are assumed to exhibit no or mild non-specific symptoms. Thus, they may contribute to the rapid and mostly silent circulation of the virus among humans. Since SARS-CoV-2 can be detected in stool samples it has recently been proposed to monitor SARS-CoV-2 in wastewaters (WW) as a complementary tool to investigate virus circulation in human populations. In the present work we assumed that the quantification of SARS-CoV-2 genomes in wastewaters should correlate with the number of symptomatic or non-symptomatic carriers. To test this hypothesis, we performed a time-course quantitative analysis of SARS-CoV-2 by RT-qPCR in raw wastewater samples collected from several major wastewater treatment plant (WWTP) of the Parisian area. The study was conducted from 5 March to 23 April 2020, therefore including the lockdown period in France (since 17 March 2020). We confirmed that the increase of genome units in raw wastewaters accurately followed the increase of human COVID-19 cases observed at the regional level. Of note, the viral genomes could be detected before the beginning of the exponential growth of the epidemic. As importantly, a marked decrease in the quantities of genomes units was observed concomitantly with the reduction in the number of new COVID-19 cases which was an expected consequence of the lockdown. A s a conclusion, this work suggests that a quantitative monitoring of SARS-CoV-2 genomes in wastewaters should bring important and additional information for an improved survey of SARS-CoV-2 circulation at the local or regional scale.list the conclusions

Existem diversas técnicas para a detecção quantitativa e qualitativa do SARS-CoV-2. Entre essas diferentes metodologias, qual é dita como o padrão-ouro para o diagnóstico da covid-19?A. Quimioluminescência.B. Imunocromatografia.C. RT-PCR.

Which of the following statements is true about diagnostic testing for viruses Group of answer choicesSerology can only be used to determine exposure to a virus, not immunityPCR is commonly used to determine if a patient is infectiousWhole genome sequencing is useful for evaluating viral transmissionSample collection does not influence PCR assay performanceNone of the above are true