How are antibody-antigen reactions visualized in an ELISA test?Multiple choice question.Color change of substrateObservation with fluorescence microscopeChanges in amount of radioactivity in sampleBands on filter paper
Question
How are antibody-antigen reactions visualized in an ELISA test?Multiple choice question.Color change of substrateObservation with fluorescence microscopeChanges in amount of radioactivity in sampleBands on filter paper
Solution
The visualization of antibody-antigen reactions in an ELISA test is done through a color change of the substrate. This color change is a result of an enzymatic reaction and can be measured using a spectrophotometer. The intensity of the color is proportional to the amount of antigen in the sample. Therefore, the correct answer is "Color change of substrate".
Similar Questions
In immunofluorescence testing, a fluorescent is attached to a specific antibody to provide a sensitive test for the presence of a specific antigen or antibody.
When an antigen-antibody reaction involves whole cells, the reaction can be observed Blank______.Multiple choice question.by detecting radioactive decayby looking for a color changewith a light microscope
Which of the following uses a known fluorescent antibody to detect the presence of a target antigen?Multiple choice question.Direct antigen testRadioimmunoassayIndirect immunofluorescence testDirect immunofluorescence testDirect ELISACapture ELISA
As opposed to the , the ELISA is a quantitative method.
Distinguish between antigens and antibodies and describe the roles of antibodies.
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