Cell numbers can be counted directly by all of the following except A) microscope counting chamber. B) Coulter counter. C) flow cytometer. D) turbidity
Question
Cell numbers can be counted directly by all of the following except
A) microscope counting chamber. B) Coulter counter. C) flow cytometer. D) turbidity
Solution
The correct answer is D) turbidity.
Turbidity is a measure of the cloudiness or haziness of a fluid caused by large numbers of individual particles that are generally invisible to the naked eye. It does not count cell numbers directly, but rather gives an estimate of the concentration of cells based on how much light is scattered by a suspension of cells.
On the other hand, a microscope counting chamber (A), a Coulter counter (B), and a flow cytometer (C) are all instruments that can directly count the number of cells in a sample.
A microscope counting chamber is a thick glass microscope slide with a grid of etched squares of known size. The depth of the chamber is also known, so you can count the number of cells in a certain volume of fluid.
A Coulter counter works by drawing cells through a small hole and measuring changes in electrical conductivity.
A flow cytometer can count cells and sort them based on their properties as they flow past a laser.
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