Kaya is investigating the effectiveness of different antiseptics on the growth of E. coli bacterium.The are testing three antiseptics at 50% concentration.This is part of Kaya's method:8. Using sterilised forceps, dip a paper disc in antiseptic one (figure 1).9. Shake off any excess liquid.10. Lift the lid of the agar plate slightly, then place the paper disc down in the relevant section of the agar plate (figure 2).11. Repeat steps 8 to 10 with the other two antiseptics, using a new pair of sterilised forceps each time.How could Kaya improve the accuracy of this part of the method, to ensure each disc had the same amount of antiseptic on it?Choose from the following.Use sterilised pipettes to place a set number of drops of the disinfectants on each paper disc.Make sure the antiseptics are all the same concentration.Dip a fourth disc in another disinfectant, so four are tested at the same time.Make sure the antiseptics are creams, and not liquids.I DON'T KNOWSUBMIT ANSWER

Zoe is investigating the effectiveness of different antiseptics on the growth of E. coli bacterium.She is testing three antiseptics at 5% concentration.This is part of Zoe's method:8. Using sterilised forceps, dip a paper disc in antiseptic one (figure 1).9. Shake off any excess liquid.10. Lift the lid of the agar plate slightly, then place the paper disc down in the relevant section of the agar plate (figure 2).11. Repeat steps 8 to 10 with the other two antiseptics, using a new pair of sterilised forceps each time.How could Zoe improve the accuracy of this part of the method, to ensure each disc has the same amount of antiseptic on it?Choose from the following.Dip a fourth disc in another disinfectant, so four are tested at the same time.Shake the discs for a set amount of time.Only dip half of each disc in the disinfectants.Use sterilised pipettes to place a set number of drops of the disinfectants on each paper disc.I DON'T KNOWSUBMIT ANSWERLearning MaterialChoose your materialCENTURY ExplainsSlideshowAim & BackgroundVariablesEquipment IEquipment IIAutoclave & IncubatorSafetyResults TableCalculator FunctionMethod: Clean the Work SurfaceMethod: Bunsen BurnerMethod: Agar Plate LabelsMethod: ControlMethod: Disc 1Method: Disc LocationMethod: Control DiscMethod: Seal with TapeAnalysis: Zone of InhibitionAnalysis: Mean Zone MeasurementsAnalysis: Calculate Mean Cross-sectional AreaConclusionAlternative Method IAlternative Method IIQuestionQuestionQuestionQuestionQuestionQuestionQuestionQuestionQuestionQuestionQuestionQuestionSummary

The images below show the main steps involved when investigating the effect of antiseptics on the growth of bacteria.Place the images in the correct order.Step 1Step 2Step 3Step 4Step 5Step 6Possible AnswersI DON'T KNOWRESETSUBMIT ANSWERLearning MaterialChoose your materialCENTURY ExplainsSlideshowAim & BackgroundVariablesEquipment IEquipment IIAutoclave & IncubatorSafetyResults TableCalculator FunctionMethod: Clean the Work SurfaceMethod: Bunsen BurnerMethod: Agar Plate LabelsMethod: ControlMethod: Disc 1Method: Disc LocationMethod: Control DiscMethod: Seal with TapeAnalysis: Zone of InhibitionAnalysis: Mean Zone MeasurementsAnalysis: Calculate Mean Cross-sectional AreaConclusionAlternative Method IAlternative Method IIQuestionQuestionQuestionQuestionQuestionQuestionQuestionQuestionQuestionQuestionQuestionQuestionSummary

When investigating the effect of antimicrobials on the growth of bacteria, it is important that the antimicrobials are only tested on one type of bacteria. Therefore, it is essential that aseptic technique is used when carrying out this required practical.Below are methods of aseptic technique that should be used when carrying out this practical.Match each method to the reason why it is carried out.Do not place tape all the way around the lid of the agar plate.Secure the lid of the agar plate with tape.Incubate the agar plate at 25 °C for 48 hours.Keep the agar plate and other equipment close to the Bunsen burner.Possible AnswersTo prevent the lid from coming off and the culture from becoming contaminated with airborne microorganisms.To prevent the lid from coming off and the bacteria from contaminating the air.To allow oxygen in, preventing the growth of dangerous, anaerobic bacteria.To ensure they are close to the updraft created by the flame, which will limit contamination by airborne microorganisms and dust particles.To allow oxygen out, preventing the growth of dangerous, anaerobic bacteria.To prevent the growth of human pathogens which thrive at body temperature.To ensure they are close to the updraft created by the flame, which will make it easier when dipping the discs in the antiseptic.I DON'T KNOWRESETSUBMIT ANSWER

A student carried out an experiment, looking at the effect of different antiseptics on the growth of the bacterium, Micrococcus luteus.Their results are shown below:The student made the following conclusions:1. Antiseptic 1 is the least effective at killing or stopping the growth of bacteria.2. Antiseptic 3 has the smallest zone of inhibtion.3. Antiseptic 1 is more effective than antiseptic 2.Which of the following conclusions are correct?1 & 21, 2 & 31 & 32 & 3

A student carried out an experiment, looking at the effect of different antiseptics on the growth of the bacterium, Micrococcus luteus.Their results are shown below:The student made the following conclusions:1. Antiseptic 1 has the smallest zone of inhibition.2. Antiseptic 2 is the most effective at killing or stopping the growth of bacteria.3. Antiseptic 1 is more effective than antiseptic 3.Which of the following conclusions are correct?1 & 31 & 22 & 31, 2 & 3