Below is the position of primer binding sites on a sequence of DNA that is to be amplified using PCR. Which pair of primers will amplify this sequence?5’ GCATCAATCT …. AAGCTACCGA 3’Group of answer choices5’ AGATTGATGC 3’ and 5’ AAGCTACCGA 3’5’ GCATCAATCT 3' and 5' AAGCTACCGA 3’5’ AGATTGATGC 3’ and 5’ TCGGTAGCTT 3’5’ GCATCAATCT 3’ and 5’ TCGGTAGCTT 3’
Question
Below is the position of primer binding sites on a sequence of DNA that is to be amplified using PCR. Which pair of primers will amplify this sequence?5’ GCATCAATCT …. AAGCTACCGA 3’Group of answer choices5’ AGATTGATGC 3’ and 5’ AAGCTACCGA 3’5’ GCATCAATCT 3' and 5' AAGCTACCGA 3’5’ AGATTGATGC 3’ and 5’ TCGGTAGCTT 3’5’ GCATCAATCT 3’ and 5’ TCGGTAGCTT 3’
Solution
The correct pair of primers that will amplify this sequence is 5’ GCATCAATCT 3' and 5' AAGCTACCGA 3’.
Here's why:
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Primers are short pieces of DNA that are made in a laboratory. Since they're custom built, primers can have any sequence of nucleotides you'd like.
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In a PCR reaction, the primers are designed to bind to the DNA sequence at specific locations where the amplification should start.
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Each primer binds at the start of the sequence to be amplified. One primer binds at the start of the sequence on one strand of DNA, and the other primer binds at the start of the sequence on the other strand of DNA.
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The primers used in PCR are typically 20-30 bases long, and they need to match the start of the sequence to be amplified.
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In this case, the primer 5’ GCATCAATCT 3' will bind to the start of the sequence on one strand of DNA, and the primer 5' AAGCTACCGA 3’ will bind to the start of the sequence on the other strand of DNA.
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Therefore, these are the correct primers to use for the amplification of this specific DNA sequence.
Similar Questions
A scientist designs a primer, which is a short oligonucleotide, for use in a DNA sequencing reaction. The primer is complementary to the DNA template shown below.3’-AGCTAGCGATCGGACGAT-5’Which of the following shows the sequence and orientation of the primer?Choose 1 answer:Choose 1 answer:(Choice A) 3’-TCGATCGCTAGCCTGCTA-5’A3’-TCGATCGCTAGCCTGCTA-5’(Choice B) 5’-TCGATCGCTAGCCTGCTA-3’B5’-TCGATCGCTAGCCTGCTA-3’(Choice C) 5’-CTAGCTATCGATTCATCG-3’C5’-CTAGCTATCGATTCATCG-3’(Choice D) 3’-CTAGCTATCGATTCATCG-5’D3’-CTAGCTATCGATTCATCG-5’
Organize the following steps of a PCR cycle in the correct sequence.The mixture is cooled to allow the binding of primers to the end of target DNA sequences.Free nucleotides are added in the 5’ to 3’ direction by the Taq DNA polymerase.The reaction mixture is heated to separate the double-stranded DNA.Group of answer choices1, 2, 31, 3, 22, 1, 32, 3, 13, 1, 23, 2, 1
Which of the following statements best describes the amplification of DNA in each PCR cycle?Group of answer choicesThe number of double stranded DNA molecules produced that contain the target sequence DOUBLES with each PCR cycleThe number of double stranded DNA molecules produced that contain the target sequence INCREASES BY 2 molecules with each PCR cycleThe number of double stranded DNA molecules produced that contain the target sequence INCREASES BY 1 molecule with each PCR cycleThe number of double stranded DNA molecules produced that contain the target sequence REMAINS CONSTANT with each PCR cycle
After a polymerase chain reaction (PCR), agarose gel electrophoresis is often used to:Group of answer choicesverify that the desired DNA sequence has been amplifiedamplify the DNAconvert cDNA into genomic DNAconvert cDNA into messenger RNAsynthesize primer DNA molecules
Which of the following 10-base primers would base-pair with this single-stranded DNA sequence?3' GATCGTAACTACAGGCGTAGCTGACGTGTCCASelect all that apply.Group of answer choices3' GATCGTAACT5' GATCGTAACT3' CTAGCATTGA5' CTAGCATTGA5' GACGTGTCCA
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