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Give a single base pair substitution (write out the codon before and after mutation) that could cause a truncation of this peptide. If there are multiple ways, you only need to identify one. (1 mark)

Question

Give a single base pair substitution (write out the codon before and after mutation) that could cause a truncation of this peptide. If there are multiple ways, you only need to identify one. (1 mark)

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Solution

To answer this question, I need to know the sequence of the peptide or the DNA/RNA sequence that codes for it. A truncation mutation usually occurs when a base pair substitution results in a premature stop codon, which halts protein synthesis.

For example, if we have a codon sequence like GAA which codes for the amino acid Glutamate, a single base pair substitution changing it to UAA would result in a stop codon, truncating the peptide.

So, the codon before mutation is GAA (Glutamate) and after mutation is UAA (Stop).

Please note that the actual codon sequence may vary depending on the specific peptide sequence you are referring to.

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