The correct option in the context of Gram staining is: Primary stain – Crystal violet, Destainer – Alcohol, Counterstain – Safranin. Here's why: 1. Primary stain: The primary stain used in Gram staining is crystal violet. This stain is applied to a heat-fixed smear of bacterial culture. All cells

Choose the correct option in context of Gram staining Primary stain – Crystal violet, Destainer – Peptidoglycan, Counterstain – Eosin Primary stain – Safranin, Destainer – Water, Counterstain – Crystal violet Primary stain – Eosin, Destainer – Water, Counterstain – Crystal violet Primary stain – Crystal violet, Destainer – Alcohol, Counterstain – Safranin

Question

Choose the correct option in context of Gram staining Primary stain – Crystal violet, Destainer – Peptidoglycan, Counterstain – Eosin Primary stain – Safranin, Destainer – Water, Counterstain – Crystal violet Primary stain – Eosin, Destainer – Water, Counterstain – Crystal violet Primary stain – Crystal violet, Destainer – Alcohol, Counterstain – Safranin

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Solution 1

The correct option in the context of Gram staining is: Primary stain – Crystal violet, Destainer – Alcohol, Counterstain – Safranin.

Here's why:

Primary stain: The primary stain used in Gram staining is crystal violet. This stain is applied to a heat-fixed smear of bacterial culture. All cells

Solution 2

The correct option in the context of Gram staining is "Primary stain – Crystal violet, Destainer – Alcohol, Counterstain – Safranin".

Here's why:

Primary stain: The primary stain used in Gram staining is crystal violet. This stain is applied to a heat-fixed smear of bacterial culture. All cells are stained by crystal violet.
Destainer: The destainer used is alcohol or acetone (not peptidoglycan or water). This step is critical as it differentiates gram-positive bacteria (which retain the crystal violet stain) from gram-negative bacteria (which do not).
Counterstain: The counterstain used is safranin (not eosin or crystal violet). After the destaining step, the gram-negative bacteria are colorless and the counterstain (safranin) is applied to give them a pink color. The gram-positive bacteria, which have retained the crystal violet stain, are not affected by the counterstain.

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