Select which of the following statements are true for gas chromatography.Group of answer choicesCapillary columns have high efficiency and so can separate many analytes in a sample, but are easily overloaded.Samples for gas chromatography must be gases at room temperature.A flame ionisation detector can identify the molecular structure of a compound eluting from a column.The column temperature is often increased during a gas chromatographic analysis.The mobile phase is an inert gas such as argon or krypton
Question
Select which of the following statements are true for gas chromatography.Group of answer choicesCapillary columns have high efficiency and so can separate many analytes in a sample, but are easily overloaded.Samples for gas chromatography must be gases at room temperature.A flame ionisation detector can identify the molecular structure of a compound eluting from a column.The column temperature is often increased during a gas chromatographic analysis.The mobile phase is an inert gas such as argon or krypton
Solution
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True - Capillary columns in gas chromatography do have high efficiency and can separate many analytes in a sample. However, they can be easily overloaded due to their small diameter.
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False - Samples for gas chromatography do not necessarily have to be gases at room temperature. They can be volatile liquids or solids that can be vaporized without decomposition.
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False - A flame ionization detector (FID) in gas chromatography can detect the presence of organic compounds, but it cannot identify the molecular structure of a compound eluting from a column.
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True - The column temperature is often increased during a gas chromatographic analysis. This is known as temperature programming, which helps in the separation of different components in the sample.
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False - The mobile phase in gas chromatography is typically an inert gas such as helium or nitrogen, not argon or krypton. These gases are used because they do not react with the sample components.
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The basic principle involved in gas-solid chromatography is Answer chromatography.
Select which of the following statements are true for high performance liquid chromatography (HPLC)Group of answer choicesHPLC often has an injector that only allows a fixed volume of sample (e.g. 10 μL) to be injected.There is no low-cost detector for HPLC that is as general, as sensitive, and with as large a relative range as the flame ionisation detector in gas chromatography.If the compounds in a normal phase HPLC analysis using a 50:50 vol/vol mixture of hexane: ethyl acetate were eluting too slowly, increasing the concentration of hexane would decrease the retention times of the analytes.The general elution problem in HPLC is solved by using rapid changes in temperature of the column and mobile phase.An HPLC column containing very small, uniform spherical packing material will have a higher column efficiency than a column with the particles of the same material that are larger and more irregular in size and shape.
One disadvantage of gas chromatography is cannot yield quantitative results.Question 15Select one:TrueFalse
Gas chromatography can separate compounds based on which properties?
The gas-solid chromatography is ______________ chromatography as per basic principle involved.a.Adsorptionb.Absorptionc.Exclusiond.Ion-exchange
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