You have a solution containing 10mg/ml of protein bovine serum albumin (BSA). MW of BSA is 68000. Calculate the concentration of this solution expressed as mM (Express your answer to two decimal places, don't forget the unit symbol, do not place a space between the numerical value and the unit symbol in your answer).
Question
You have a solution containing 10mg/ml of protein bovine serum albumin (BSA). MW of BSA is 68000. Calculate the concentration of this solution expressed as mM
(Express your answer to two decimal places, don't forget the unit symbol, do not place a space between the numerical value and the unit symbol in your answer).
Solution
To calculate the concentration of the solution in mM (millimolar), we first need to convert the mass concentration (mg/ml) to molar concentration (mol/L).
- Convert the mass of BSA from mg to g: 10 mg/ml = 0.01 g/ml
- Calculate the number of moles in 0.01 g of BSA: 0.01 g / 68000 g/mol = 1.47 x 10^-7 mol
- Convert the volume from ml to L: 1 ml = 0.001 L
- Calculate the molar concentration: 1.47 x 10^-7 mol / 0.001 L = 1.47 x 10^-4 M
- Convert the molar concentration from M to mM: 1.47 x 10^-4 M = 0.147 mM
So, the concentration of the solution is 0.147mM.
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