Among individuals in a human population, minor variations exist in nucleotidesequences of chromosomes. These variations can lead to gain or loss of sites forspecific restriction enzymes. Which of the following technique is used to identifysuch variations?(A) Polymerase dependent fragment insertion(B) Real-time polymerase chain reaction(C) Restriction fragment length polymorphism(D) Reverse transcriptase polymerase chain reaction
Question
Among individuals in a human population, minor variations exist in nucleotidesequences of chromosomes. These variations can lead to gain or loss of sites forspecific restriction enzymes. Which of the following technique is used to identifysuch variations?(A) Polymerase dependent fragment insertion(B) Real-time polymerase chain reaction(C) Restriction fragment length polymorphism(D) Reverse transcriptase polymerase chain reaction
Solution
The technique used to identify variations in nucleotide sequences of chromosomes, which can lead to gain or loss of sites for specific restriction enzymes, is (C) Restriction fragment length polymorphism.
Here's a step-by-step explanation:
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Restriction fragment length polymorphism (RFLP) is a technique that exploits variations in homologous DNA sequences.
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In this technique, DNA samples are collected and then digested by restriction enzymes, which cut the DNA at specific sequences.
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The resulting DNA fragments are then separated by gel electrophoresis, a process that separates the fragments based on their size.
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The separated fragments are then transferred to a membrane and hybridized with a radioactive probe that binds to the DNA sequences of interest.
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The pattern of bands on the membrane reflects the differences in the DNA sequences among individuals.
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Therefore, RFLP can be used to identify variations in nucleotide sequences of chromosomes in a human population.
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