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Below is a sequence of DNA that is going to be replicated. This is the sequence of the leading strand and only shows the position at which primase binds to synthesise RNA primers. Based on this sequence, which RNA primers would allow replication from both the leading and lagging strands?5’ ….TTATCGCTGA….AGAGGACGTA….CATTGACAGG 3’Group of answer choicesFrom the leading strand: 5’ UUAUCGCUGA 3’ and 5’ AGAGGACGUA 3’From the lagging strand: 5’ CCUGUCAAUG 3’From the leading strand: 5’ CAUUGACAGG 3’From the lagging strand: 5’ UCAGCGAUAA 3’ and 5’ UACGUCCUCU 3’From the leading strand: 5’ UCAGCGAUAA 3’ and 5’ UACGUCCUCU 3’From the lagging strand: 5’ CAUUGACAGG 3’From the leading strand: 5’ CCUGUCAAUG 3’From the lagging strand: 5’ UUAUCGCUGA 3’ and 5’ AGAGGACGUA 3’

Question

Below is a sequence of DNA that is going to be replicated. This is the sequence of the leading strand and only shows the position at which primase binds to synthesise RNA primers. Based on this sequence, which RNA primers would allow replication from both the leading and lagging strands?5’ ….TTATCGCTGA….AGAGGACGTA….CATTGACAGG 3’Group of answer choicesFrom the leading strand: 5’ UUAUCGCUGA 3’ and 5’ AGAGGACGUA 3’From the lagging strand: 5’ CCUGUCAAUG 3’From the leading strand: 5’ CAUUGACAGG 3’From the lagging strand: 5’ UCAGCGAUAA 3’ and 5’ UACGUCCUCU 3’From the leading strand: 5’ UCAGCGAUAA 3’ and 5’ UACGUCCUCU 3’From the lagging strand: 5’ CAUUGACAGG 3’From the leading strand: 5’ CCUGUCAAUG 3’From the lagging strand: 5’ UUAUCGCUGA 3’ and 5’ AGAGGACGUA 3’

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Solution

The correct answer would be:

From the leading strand: 5’ UUAUCGCUGA 3’ and 5’ AGAGGACGUA 3’

From the lagging strand: 5’ UCAGCGAUAA 3’ and 5’ UACGUCCUCU 3’

Here's why:

DNA replication is semi-conservative and occurs in the 5' to 3' direction. The leading strand is synthesized continuously in the 5' to 3' direction, while the lagging strand is synthesized discontinuously in the 5' to 3' direction, but overall in the 3' to 5' direction.

The RNA primers for the leading strand would be complementary to the DNA sequence given, and would also be synthesized in the 5' to 3' direction. Therefore, the RNA primers for the leading strand would be 5’ UUAUCGCUGA 3’ and 5’ AGAGGACGUA 3’.

The RNA primers for the lagging strand would also be complementary to the DNA sequence given, but because the lagging strand is synthesized in the 3' to 5' direction, the RNA primers would be synthesized in the opposite direction to the DNA sequence given. Therefore, the RNA primers for the lagging strand would be 5’ UCAGCGAUAA 3’ and 5’ UACGUCCUCU 3’.

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