What are three key proteins required during DNA unwinding at the replication fork?Group of answer choicesDNA polymerase, DNA gyrase, DNA ligaseDNA helicase, DNA gyrase, single stranded DNA binding proteinDNA helicase, DNA gyrase, DNA polymeraseDNA polymerase, DNA gyrase, DNA ligase PreviousNext
Question
What are three key proteins required during DNA unwinding at the replication fork?Group of answer choicesDNA polymerase, DNA gyrase, DNA ligaseDNA helicase, DNA gyrase, single stranded DNA binding proteinDNA helicase, DNA gyrase, DNA polymeraseDNA polymerase, DNA gyrase, DNA ligase PreviousNext
Solution
The three key proteins required during DNA unwinding at the replication fork are DNA helicase, DNA gyrase, and single stranded DNA binding protein.
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DNA helicase: This enzyme is responsible for unwinding the double-stranded DNA helix into two single strands which can then be replicated. It does this by breaking the hydrogen bonds between the base pairs.
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DNA gyrase: This enzyme is a type of topoisomerase that relieves the tension in the DNA molecule that is created by the unwinding process. It does this by introducing negative supercoils into the DNA.
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Single stranded DNA binding protein: These proteins bind to the single-stranded DNA to prevent the strands from reannealing and to protect them from degradation. They also help in keeping the strands apart for replication to occur.
Similar Questions
The enzyme which unwinds short stretches of DNA helix immediately ahead of a replication fork is:Select one:a. Helicaseb. DNA polymerasec. Topoisomerased. DNA Gyrase
Which of these types of enzymes is involved in setting up the replication fork?Group of answer choicesDNA glycosylaseDNA helicaseTelomerasePrimase
The following are enzymes required in DNA replication except A. DNA helicase B. Topoisomerase C. DNA primase D. DNA synthase
A. DNA polymerase B. RNA polymerase C. Topoisomerase D. Helicase E. DNA ligase 12.____ catalyzes DNA synthesis in a 5' to 3' direction, adding dNTPs to the 3' end. 13.____which replication enzyme unwinds and separates the DNA strands? 14____ releases tension in supercoils of DNA 15.____ Fills gaps between Okazaki fragments in lagging strand 16.____ Joins gaps in DNA
Which is true during DNA replication?Group of answer choicesDNA ligase is the last step of the polymerisation reaction for the lagging strand, but is not used on the leading strand.DnaA binds to regions that are rich in AT as this purine-purine base pairing forms only two hydrogen bonds.The β sliding clamp prevents the dissociation of DNA polymerase III, increasing the length of DNA each polymerase can synthesise.DNA gyrase binds to and applies tension to the lagging strand.
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