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Which of the following principles is used to help with drug detection in mass spectrometry?Question 3Answera.Fragmentation of molecule in the mass spectrometerb.Hydrolysis of a drug metabolite before analysisc.All the aboved.Derivatization of the molecule before analysis

Question

Which of the following principles is used to help with drug detection in mass spectrometry?Question 3Answera.Fragmentation of molecule in the mass spectrometerb.Hydrolysis of a drug metabolite before analysisc.All the aboved.Derivatization of the molecule before analysis

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Solution

The principles used to help with drug detection in mass spectrometry include:

a. Fragmentation of molecule in the mass spectrometer: This process involves breaking down the molecule into smaller pieces. This is done to make the molecule easier to identify and analyze. The fragments are then separated based on their mass-to-charge ratio.

b. Hydrolysis of a drug metabolite before analysis: This process involves breaking down the drug metabolite into its constituent parts using water. This is done to make the metabolite easier to identify and analyze.

d. Derivatization of the molecule before analysis: This process involves chemically modifying the molecule to make it more detectable or to enhance its detection. This is done to improve the sensitivity and selectivity of the analysis.

Therefore, the answer is c. All the above. All these principles are used in mass spectrometry for drug detection.

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