which dye is generally used in real time pcr?

The dye generally used in real-time PCR (Polymerase Chain Reaction) is SYBR Green. This dye binds to the minor groove of the DNA double helix. When bound to DNA, SYBR Green's fluorescence is enhanced, allowing the detection and quantification of the amount of DNA present during the PCR process. This is useful in real-time PCR as it allows the monitoring of the amplification process in real time.