Which is true of the 5' - 3' exonuclease activity of DNA polymerase I?Group of answer choicesIt proofreads the DNA replication process of polymerase IIt can remove RNA primers via hydrolysis of their phosphodiester backbone linkages.It proofreads the DNA replication process of polymerase IIIIt is involved in synthesis of Okazaki fragments on the lagging strand.
Question
Which is true of the 5' - 3' exonuclease activity of DNA polymerase I?Group of answer choicesIt proofreads the DNA replication process of polymerase IIt can remove RNA primers via hydrolysis of their phosphodiester backbone linkages.It proofreads the DNA replication process of polymerase IIIIt is involved in synthesis of Okazaki fragments on the lagging strand.
Solution
The correct answer is: It can remove RNA primers via hydrolysis of their phosphodiester backbone linkages.
Here's why:
DNA Polymerase I has a 5' - 3' exonuclease activity that is involved in the removal of RNA primers from the newly synthesized DNA strand. During DNA replication, RNA primers are initially added to the DNA template strand to provide a starting point for the DNA polymerase to begin synthesis. Once a segment of DNA has been replicated, DNA Polymerase I comes in and removes these RNA primers. It does this by hydrolyzing the phosphodiester bonds that link the RNA nucleotides together, effectively removing the RNA primer from the DNA strand. This is a crucial step in DNA replication as it ensures that the final, replicated DNA molecule is composed entirely of DNA, not RNA.
Similar Questions
Which is true of the exonuclease activity of DNA polymerase III?Group of answer choicesIt is used to replace an RNA nucleotide from the primer sequence with a DNA nucleotide.It is involved in phosphodiester bond hydrolysis at the 5’ end of a DNA strand.It moves in the 5’ to 3’ direction on the replicated strandThe product of its enzymatic activity is dAMP, dCMP, dGMP or dTMP.
The proofreading activity, to maintain the fidelity of DNA synthesis,Group of answer choicesis a function of the 3'→5' exonuclease activity of DNA polymerasesis a function of the 5'→3' exonuclease activity of DNA polymerase IIIoccurs only when the DNA polymerase on the lagging strand encounters the previously synthesised fragmentoccurs after the DNA replication process has been completed
Which of the following reactions is required for proofreading during DNA replication by DNA polymerase III?Group of answer choices5'→3' exonuclease activity3'→5' endonuclease activity5'→3' endonuclease activity3'→5' exonuclease activity
Which is true during DNA replication?Group of answer choicesDNA ligase replaces the nucleotides in the RNA primer with DNA on the newly synthesised strands.RNA primers bound to both the leading and lagging strand are degraded by 5' - 3' exonucleases.DNA polymerase III synthesises the leading strand and DNA polymerase I synthesises the lagging strandPrimase is required to make primers on the lagging strand only
Which activity of DNA polymerase I is its 'proofreading' activityGroup of answer choices5' to 3' exonuclease activityDNA polymerase I doesn't have this activityBoth 5' to 3' and 3' to 5' exonuclease activities3' to 5' exonuclease activity
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