Which of the following reactions is required for proofreading during DNA replication by DNA polymerase III?Group of answer choices5'→3' exonuclease activity3'→5' endonuclease activity5'→3' endonuclease activity3'→5' exonuclease activity
Question
Which of the following reactions is required for proofreading during DNA replication by DNA polymerase III?Group of answer choices5'→3' exonuclease activity3'→5' endonuclease activity5'→3' endonuclease activity3'→5' exonuclease activity
Solution
The reaction required for proofreading during DNA replication by DNA polymerase III is 3'→5' exonuclease activity.
Here's a step by step explanation:
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DNA polymerase III is the main enzyme involved in DNA replication. It adds nucleotides to the new DNA strand, building it in a 5' to 3' direction.
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However, DNA polymerase III can make mistakes and add the wrong nucleotide.
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To correct these mistakes, DNA polymerase III has a proofreading function. This is where the 3'→5' exonuclease activity comes in.
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If a wrong nucleotide is added, the enzyme can recognize the mistake. The 3'→5' exonuclease activity allows the enzyme to remove the incorrect nucleotide from the 3' end.
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Once the incorrect nucleotide is removed, DNA polymerase III can then add the correct nucleotide.
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This proofreading function greatly increases the accuracy of DNA replication.
Similar Questions
The proofreading activity, to maintain the fidelity of DNA synthesis,Group of answer choicesoccurs after the DNA replication process has been completedis a function of the 3'→5' exonuclease activity of DNA polymerasesis a function of the 5'→3' exonuclease activity of DNA polymerase IIIoccurs only when the DNA polymerase on the lagging strand encounters the previously synthesised fragment
Which activity of DNA polymerase I is its 'proofreading' activityGroup of answer choices5' to 3' exonuclease activityDNA polymerase I doesn't have this activityBoth 5' to 3' and 3' to 5' exonuclease activities3' to 5' exonuclease activity
Which DNA polymerase is involved in DNA replication?Group of answer choicesDNA polymerase I and DNA polymerase IIDNA polymerase I and DNA polymerase IIIDNA polymerase II and DNA polymerase IIIOnly DNA polymerase III
Which is true of the 5' - 3' exonuclease activity of DNA polymerase I?Group of answer choicesIt proofreads the DNA replication process of polymerase IIt can remove RNA primers via hydrolysis of their phosphodiester backbone linkages.It proofreads the DNA replication process of polymerase IIIIt is involved in synthesis of Okazaki fragments on the lagging strand.
E.coli has five different DNA polymerases. How many are involved in DNA replication?Group of answer choices5234
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