29. Which of the following about CRISPR-Cas9 technique is correct?1. Catalytically inactive Cas-9 (dCas9) cuts only one strand of DNA as opposed to both.2. The guide RNA used in the lab is analogous to the tracrRNA/crRNA hybrid in the adaptive immunesystem of bacteria.3. Homology directed repair can only allow for editing a single base pair.4. NHEJ can lead to mutations.A. 1, 2 and 3B. 1 and 3C. 2 and 4D. 4 onlyE. All of 1, 2, 3 and 4 are correc
Question
- Which of the following about CRISPR-Cas9 technique is correct?1. Catalytically inactive Cas-9 (dCas9) cuts only one strand of DNA as opposed to both.2. The guide RNA used in the lab is analogous to the tracrRNA/crRNA hybrid in the adaptive immunesystem of bacteria.3. Homology directed repair can only allow for editing a single base pair.4. NHEJ can lead to mutations.A. 1, 2 and 3B. 1 and 3C. 2 and 4D. 4 onlyE. All of 1, 2, 3 and 4 are correc
Solution
The correct answer is C. 2 and 4.
Explanation:
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Catalytically inactive Cas-9 (dCas9) does not cut DNA at all. It is often used for gene regulation and base editing, not for cutting DNA strands.
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The guide RNA used in the lab is indeed analogous to the tracrRNA/crRNA hybrid in the adaptive immune system of bacteria. It guides the Cas9 to the correct location in the DNA sequence.
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Homology directed repair (HDR) is not limited to editing a single base pair. It can insert, delete, or replace multiple DNA base pairs.
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Non-homologous end joining (NHEJ) can indeed lead to mutations. It is an error-prone repair pathway that often introduces insertions or deletions at the repair site.
Similar Questions
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