MATERIALS AND METHODS (5 marks)(cite resources ie Lab manual)A brief description of the experimental procedures used, instead ofexplaining in great detail the step by step procedure, simply refer to the labmanual or where to find the procedure for the experimental techniques used.What techniques were used for the microscopic analysis of yogurtprobiotics? ie staining method used, aseptic techniques, why?What technique(s) was/were used to count the number of probiotics inyogurt?What type of media was used for culturing E. coli?Note any changes made in the experiment and make a reference to the labmanual or supplemental handout.

Write short note on:(a) Microbial Fermentation and its energitics.(b) Production of Fermented beverages(c) Methods for measurement of bacterial growth.

Sample QuestionPlease choose all methods used to identify bacteria.Microscopic appearance

Q. Attempt all parts from the following. All parts carry equal marks. Justify the authenticity of the fluid mosaic model of the cell membrane. identify and briefly describe the technique used to isolate the cell organelles for detailed microscopy. Show a comparison between structural isomers and stereoisomers of glucose. The two strands of DNA are not identical. Why? Justify the specificity of enzymes for the reactions. What are the applications of bacteriophages in genetic engineering techniques? (any three). Define inflorescence and describe its major types. How green and yellow wavelengths, that are reflected by chlorophyll are absorbed by the plants? Viruses need a host cell to survive. How does a virus protect itself from the immune system of the host cell?Justify the importance of G3P in photosynthesis and respiration. Justify that the laboratory-manufactured sweeteners are left-handed sugars and cannot be metabolized by right-handed enzymes. How do autotrophic bacteria get energy? The diagram shows the structure of glycogen and the type of bonds that link the glucose molecules. a. Name the bond formed at point A. b. Name one site where glycogen is stored in the c. Suggest why it is an advantage that the glycogen molecule is branched. d. State two ways in which a cellulose molecule differs from a glycogen molecule.

The first step of the lab project was the extraction and purification of genomic DNA. What technique was used to isolate DNA molecules from the other type of molecules present in the cell? Ultra-Centrifugation Ion-Exchange chromatography Microscopy Gel Electrophoresis Cloning

The images below show the main steps involved when investigating the effect of antiseptics on the growth of bacteria.Place the images in the correct order.Step 1Step 2Step 3Step 4Step 5Step 6Possible AnswersI DON'T KNOWRESETSUBMIT ANSWERLearning MaterialChoose your materialCENTURY ExplainsSlideshowAim & BackgroundVariablesEquipment IEquipment IIAutoclave & IncubatorSafetyResults TableCalculator FunctionMethod: Clean the Work SurfaceMethod: Bunsen BurnerMethod: Agar Plate LabelsMethod: ControlMethod: Disc 1Method: Disc LocationMethod: Control DiscMethod: Seal with TapeAnalysis: Zone of InhibitionAnalysis: Mean Zone MeasurementsAnalysis: Calculate Mean Cross-sectional AreaConclusionAlternative Method IAlternative Method IIQuestionQuestionQuestionQuestionQuestionQuestionQuestionQuestionQuestionQuestionQuestionQuestionSummary