A student performed a spread plate count of a bacterial culture and recorded 32 and 48 colonies on duplicate plates of the 10-4 dilution. What will be the bacterial count (cfu/ml) in the original culture? Group of answer choices 8.0 x 105 4.0 x 107 8.0 x 106 4.8 x 105 3.2 x 105 4.0 x 106 4.8 x 107 8.0 x 107 4.0 x 105 3.2 x 106 4.8 x 106 3.2 x 107
Question
A student performed a spread plate count of a bacterial culture and recorded 32 and 48 colonies on duplicate plates of the 10-4 dilution. What will be the bacterial count (cfu/ml) in the original culture?
Group of answer choices
8.0 x 105
4.0 x 107
8.0 x 106
4.8 x 105
3.2 x 105
4.0 x 106
4.8 x 107
8.0 x 107
4.0 x 105
3.2 x 106
4.8 x 106
3.2 x 107
Solution
Para calcular el recuento bacteriano (cfu/ml) en la cultura original, sigue estos pasos:
-
Promedio de colonias: Primero, calcula el promedio de las colonias contadas en las placas duplicadas.
-
Dilución: La dilución utilizada es .
-
Recuento bacteriano: Usa la fórmula para calcular el recuento bacteriano en la cultura original:
Por lo tanto, el recuento bacteriano en la cultura original es cfu/ml.
Similar Questions
A bacterial culture contains 106 cells/mL. A serial dilution (each 1/10) was performed to confirm the numbers through a spread plate count. How many dilutions would be required to obtain a countable number of colonies on the plate (between 30-300)? Group of answer choices 4 3 6 2 5
In the enumeration of bacteria in a culture by a plate count, all of the following apply when determining the numbers except: Group of answer choices All bacteria in the culture are counted Statistically only plates with between 30-300 colonies should be counted It is assumed that each colony arose from division of one or a few cells All of the answer choices are correct The medium must contain nutrients used by the bacteria
A petri dish originally contained 140 bacteria. A scientist let the bacteria grow and now there are 870 of them. How many more bacteria are there now?
A sample taken from a sewage treatment plant is likely to have too many bacteria to count directly. What would be best done when determining the numbers of viable bacteria in the sample? Group of answer choices Filtration then spread plating the filtrate Serial dilution and spread plating the dilutions 16-streak plating of the wastewater sample All of the other answer choices are correct Optical density reading in a spectrophotometer
Bacteria from three different cultures were plated on agar plates containing ampicillin, an antibiotic. The number of colonies that grew on each plate can be seen in the graph below. If you assume that 100 bacteria were plated on each plate, what percentage of the bacteria on plate B were resistant to ampicillin?multiple choice3%0%10%100%
Upgrade your grade with Knowee
Get personalized homework help. Review tough concepts in more detail, or go deeper into your topic by exploring other relevant questions.