Experimenters isolate a biological sample believed to be composed entirely of glycine. In order to confirm their belief, they can hydrolyze the peptide and then:A.use separation techniques to determine if the sample contains amino acids with hydrophobic R groups.B.raise the pH of the sample and apply an electric field to test for the presence of zwitterions.C.titrate the sample with NaOH to determine if it contains amino acids with acidic R groups.D.use polarized light in order to determine whether or not the sample is optically active.

Ultraviolet (UV) light can be used to estimate protein solution concentrations because A. Alanine absorbs at 280 nm. B. all the amino acids absorb UV light. C. aliphatic amino acids absorb at 280 nm. D. tryptophan and tyrosine absorb at 280 nm. E. All of the above

The ion-exchange chromatography is widely used for protein separation(a) Describe the principles of ion-exchange chromatography;(b) Explain why changing the pH allows different amino acids to elute from the column at differenttimes

Describe how you would perform a chromatography experiment to produce thechromatogram shown in (c). Assume you have been given the mixture of amino acids anda suitable locating agent. You are provided with common laboratory apparatus. (3) mixture of amino acids is placed as a spot onto a (pencil) baselineplaced into a (suitable) solvent /watera locating agent is added to the (finished) chromatogram (to reveal spots)

A technique for detecting a protein in a mixture after the proteins are separated by gel electrophoresis and transferred to a membrane is called ____________________.Group of answer choicessandwich ELISAwestern blottingindirect ELISAflow cytometry

Which one of the following methods CANNOT be used to determine thesecondary structure content of a protein?(A) Circular dichroism spectroscopy(B) Fourier transform infrared spectroscopy(C) Mass spectrometry(D) X-ray crystallography