Ultraviolet (UV) light can be used to estimate protein solution concentrations because A. Alanine absorbs at 280 nm. B. all the amino acids absorb UV light. C. aliphatic amino acids absorb at 280 nm. D. tryptophan and tyrosine absorb at 280 nm. E. All of the above
Question
Ultraviolet (UV) light can be used to estimate protein solution concentrations because A. Alanine absorbs at 280 nm. B. all the amino acids absorb UV light. C. aliphatic amino acids absorb at 280 nm. D. tryptophan and tyrosine absorb at 280 nm. E. All of the above
Solution
The correct answer is D. Tryptophan and tyrosine absorb at 280 nm.
This is because the aromatic rings in the side chains of these amino acids absorb ultraviolet light at a wavelength of 280 nm. This property is used in spectrophotometry to estimate the concentration of proteins in a solution, as the amount of light absorbed is proportional to the concentration of the protein. The other options are incorrect because not all amino acids absorb UV light, and alanine and aliphatic amino acids do not specifically absorb at 280 nm.
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