Consider gel-filtration chromatograpy and select the correct statement(s).Question 3Answer1.The larger the volume a particle wanders in, the earlier it elutes.2.The total column volume equals to the sum of the internal bead volume and of the volume between the beads.3.The larger the volume a particle wanders in, the later it elutes.4.The solid phase is composed of porous beads.5.It separates the analyte components according to their size.

The correct statements are:

1. The larger the volume a particle wanders in, the later it elutes.
2. The total column volume equals to the sum of the internal bead volume and of the volume between the beads.
3. The solid phase is composed of porous beads.
4. It separates the analyte components according to their size.

Explanation:

1. In gel-filtration chromatography, smaller particles enter the pores of the beads and therefore travel a longer path, causing them to elute later. Larger particles cannot enter the pores and thus travel a shorter path, causing them to elute earlier. So, the larger the volume a particle wanders in, the later it elutes.

2. The total column volume in gel-filtration chromatography indeed equals the sum of the internal bead volume (the volume inside the beads) and the volume between the beads (also known as the interstitial volume).

3. The solid phase in gel-filtration chromatography is indeed composed of porous beads. These beads act as a sieve, separating particles based on their size.

4. Gel-filtration chromatography separates analyte components according to their size. This is because smaller molecules enter the pores of the beads and travel a longer path, while larger molecules cannot enter the pores and travel a shorter path.