The sodium-potassium (Na+/K+) pump functions like an antiporter transporting Na+ and K+ icons across membranes using ATP. This protein spans the membrane with intracellular and extracellular domains. It has a binding site for Na+, K+, and ATP. An experiment was conducted to determine the locations of these binding sites. Artificial cells were created and incubated in buffers containing ATP, ouabain (or g-strophanthin, a cardiac glycoside), Na+, and K+ in varying combinations inside and outside of the cells, as indicated in the given table. The transport of Na+ and K+ was measured to determine the activity of the Na+/K+ pump.Which conclusion is supported by the data?A. The ATP binding site of the Na+/K+ pump is located on the intracellular domain of the pump.B. Ouabain does not disrupt ATP binding to the Na+/K+ pump.C. ATP is required for the transport of Na+ and not for transport of K+.D. The ATP binding site of the Na+/K+ pump is located on the extracellular domain of the pump.
Question
The sodium-potassium (Na+/K+) pump functions like an antiporter transporting Na+ and K+ icons across membranes using ATP. This protein spans the membrane with intracellular and extracellular domains. It has a binding site for Na+, K+, and ATP. An experiment was conducted to determine the locations of these binding sites. Artificial cells were created and incubated in buffers containing ATP, ouabain (or g-strophanthin, a cardiac glycoside), Na+, and K+ in varying combinations inside and outside of the cells, as indicated in the given table. The transport of Na+ and K+ was measured to determine the activity of the Na+/K+ pump.Which conclusion is supported by the data?A. The ATP binding site of the Na+/K+ pump is located on the intracellular domain of the pump.B. Ouabain does not disrupt ATP binding to the Na+/K+ pump.C. ATP is required for the transport of Na+ and not for transport of K+.D. The ATP binding site of the Na+/K+ pump is located on the extracellular domain of the pump.
Solution
The experiment involved creating artificial cells and incubating them in buffers with different combinations of ATP, ouabain, Na+, and K+. The transport of Na+ and K+ was then measured to determine the activity of the Na+/K+ pump. Based on the data provided in the table, we can draw the following conclusions:
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The ATP binding site of the Na+/K+ pump is located on the intracellular domain of the pump. This can be inferred from the fact that when ATP was present inside the cells and ouabain was added outside, there was still transport of Na+ and K+. This suggests that ATP binding can occur even when ouabain is present outside the cells, indicating that the ATP binding site is located on the intracellular domain.
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Ouabain does not disrupt ATP binding to the Na+/K+ pump. This conclusion can be drawn from the observation that when ouabain was added outside the cells, there was still transport of Na+ and K+. If ouabain disrupted ATP binding, we would expect to see a decrease or complete inhibition of Na+ and K+ transport.
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ATP is required for the transport of Na+ and not for the transport of K+. This can be inferred from the data showing that when ATP was present inside the cells and ouabain was added outside, there was still transport of Na+ but not K+. This suggests that ATP is specifically required for the transport of Na+ but not for the transport of K+.
Based on these conclusions, the correct answer is A. The ATP binding site of the Na+/K+ pump is located on the intracellular domain of the pump.
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