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A student performed a spread plate count of a bacterial culture and recorded an average of 460 colonies after plating the culture directly on duplicate agar plates and and average of 62 colonies with the 10-1 dilution duplicate plates. What will be the bacterial count (cfu/ml) in the original culture? Group of answer choices 4600 620 6200 460 540 5400 answer oin;lu

Question

A student performed a spread plate count of a bacterial culture and recorded an average of 460 colonies after plating the culture directly on duplicate agar plates and and average of 62 colonies with the 10-1 dilution duplicate plates. What will be the bacterial count (cfu/ml) in the original culture? Group of answer choices

4600

620

6200

460

540

5400 answer oin;lu

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Solution

Para calcular el recuento bacteriano (ufc/ml) en la cultura original, se deben seguir los siguientes pasos:

  1. Identificar los datos proporcionados:

    • Promedio de colonias en las placas directas: 460 colonias.
    • Promedio de colonias en las placas con dilución 10^-1: 62 colonias.
  2. Calcular el recuento bacteriano para la dilución 10^-1:

    • La dilución 10^-1 significa que la muestra original se diluyó 10 veces.
    • Para encontrar el recuento en la muestra original, se multiplica el número de colonias por el factor de dilución.
    • Recuento bacteriano = 62 colonias * 10 = 620 ufc/ml.
  3. Verificar si se necesita usar los datos de las placas directas:

    • Las placas directas tienen un promedio de 460 colonias, pero no se diluyeron.
    • Por lo tanto, el recuento bacteriano en las placas directas es 460 ufc/ml.
  4. Comparar los resultados:

    • Recuento bacteriano de las placas directas: 460 ufc/ml.
    • Recuento bacteriano de las placas con dilución 10^-1: 620 ufc/ml.
  5. Seleccionar el valor más confiable:

    • Generalmente, se prefiere el recuento de las diluciones que caen dentro de un rango contable adecuado (30-300 colonias por placa).
    • En este caso, el recuento de 62 colonias en la dilución 10^-1 es más confiable que el recuento directo de 460 colonias.

Por lo tanto, el recuento bacteriano en la cultura original es 620 ufc/ml.

La respuesta correcta es: 620.

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